General Information

    • DRAMP ID

    • DRAMP02441

    • Peptide Name

    • Gramicidin S

    • Source

    • Bacillus brevis

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • VXLFPVXLFP

    • Sequence Length

    • 10

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.29499484] Gram-positive bacterium:Staphylococcus aureus subsp.aureus CIP 4.83 ATCC 6538 (MIC=3.125μg/mL),Bacillus subtilis subsp. subtilis CIP 52.65 (MIC=3.125μg/ml),Enterococcus faecalis CIP 103015 (MIC=3.125μg/ml),Enterococcus faecalis CIP 103214 (MIC=3.125μg/ml),Bacillus cereus CIP 66.24 (MIC=6.25μg/ml),Streptococcus salivarius CIP 102503 (MIC=3.125μg/ml),Enterococcus faecium CIP 103014 (MIC=3.125μg/ml);
      • Gram-negative bacterium:Escherichia coli CIP 53.126 ATCC 8739 (MIC=12.5μg/ml),Pseudomonas aeruginosa CIP 82.118 ATCC 9027 (MIC>=100μg/ml)

    • Hemolytic Activity

      • [Ref.29499484] It has 74% hemolysis at 50μg/ml, 83% hemolysis at 100μg/ml against human red blood cells.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • The 'O' in sequence is Ornithine

    • Stereochemistry

    • Mixed(D-Phe)

    • Structure

    • Beta sheet and beta-turn

    • Structure Description

    • The peptide have the C2-symmetrical sequence [cyclo-(Val-Orn-Leu-D-Phe-Pro)2]. The two sequences Val-Orn-Leu form an antiparallel

    • Helical Wheel Diagram

    • DRAMP02441 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02441.

Physicochemical Information

    • Formula

    • C50H70N8O7

    • Absent Amino Acids

    • ACDEGHIKMNQRSTWY

    • Common Amino Acids

    • FLPVX

    • Mass

    • 1153.85

    • PI

    • 5.49

    • Basic Residues

    • 0

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • 0

    • Boman Index

    • 23.88

    • Hydrophobicity

    • 1.84

    • Aliphatic Index

    • 136

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 0

DRAMP02441

Comments Information

    • Function

    • Antibacterial activity against the Gram-negative bacteria and Gram-positive bacteria. Has hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • β,γ-diamino acids as building blocks for new analogues of Gramicidin S: Synthesis and biological activity

    • Reference

    • Eur J Med Chem. 2018 Apr 10;149:122-128.

    • Author

    • Wan Y, Stanovych A, Gori D, Zirah S, Kouklovsky C, Alezra V.