General Information
-
DRAMP ID
- DRAMP02742
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Peptide Name
- Defensin-like turtle egg white protein TEWP (TEWP; Reptiles, animals)
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Source
- Caretta caretta (Loggerhead sea turtle)
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Family
- Belongs to the beta-defensin family
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Gene
- Not found
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Sequence
- QKKCPGRCTLKCGKHERPTLPYNCGKYICCVPVKVK
-
Sequence Length
- 36
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UniProt Entry
- P0CAP0
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Protein Existence
- Protein level
Activity Information
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antiviral
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Target Organism
-
- Gram-negative bacteria: Escherichia coli (IC50=3.3 µM), Salmonella typhimurium (IC50=2.8 µM);
- Gram-positive bacterium: Staphylococcus aureus (IC50=5. 1µM).
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
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Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Beta strand (2 strands; 2 residues)
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Structure Description
- Three-dimensional structure of TEWP was determined by distance geometry and simulated annealing. The protein has no α-helix and consists of two antiparallel β-sheets. The secondary structure is consistent with the previously obtained circular dichroism spectra.
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Helical Wheel Diagram
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PDB ID
- 2B5B resolved by NMR.
- 2B5B-> 
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Predicted Structure
- There is no predicted structure for DRAMP02742.
Physicochemical Information
-
Formula
- C177H297N53O45S6
Absent Amino Acids
- ADFMSW
Common Amino Acids
- K
Mass
- 4079.99
PI
- 9.54
Basic Residues
- 10
Acidic Residues
- 1
Hydrophobic Residues
- 6
Net Charge
- +9
-
Boman Index
- -60.38
Hydrophobicity
- -0.608
Aliphatic Index
- 56.67
Half Life
-
- Mammalian:0.8 hour
- Yeast:10 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 3355
Absorbance 280nm
- 95.86
Polar Residues
- 14
DRAMP02742
Comments Information
Function
- The protein showes strong antibacterial activity against Escherichia coli and Salmonella typhimurium and also showes significant antiviral activity against an enveloped rhabdovirus, Chandipura virus, which is an emerging human pathogen.
Tissue specificity
- Detected in egg white (at protein level).
Literature Information
- ·Literature 1
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Title
- Small cationic protein from a marine turtle has beta-defensin-like fold and antibacterial and antiviral activity.
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Pubmed ID
- 16700051
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Reference
- Proteins. 2006 Aug 1;64(2):524-531.
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Author
- Chattopadhyay S, Sinha NK, Banerjee S, Roy D, Chattopadhyay D, Roy S.