General Information

    • DRAMP ID

    • DRAMP02770

    • Peptide Name

    • Pilosulin 3 (ants, insects, animals)

    • Source

    • Myrmecia banksi (Jack jumper ant) (Australian jumper ant)

    • Family

    • Belongs to the pilosulin family

    • Gene

    • Not found

    • Sequence

    • IIGLVSKGTCVLVKTVCKKVLKQG

    • Sequence Length

    • 24

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.15246874]Gram-negative bacteria: Escherichia coli (MIC<25 µM);
      • Gram-positive bacteria: Staphylococcus aureus(MIC<25 µM), Bacillus subtilis (MIC<50 µM).

    • Hemolytic Activity

      • [Ref.15246874]Non-hemolytic activity at 50 μM against human whole blood

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • IgE

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02770 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02770.

Physicochemical Information

    • Formula

    • C113H208N30O29S2

    • Absent Amino Acids

    • ADEFHMNPRWY

    • Common Amino Acids

    • KV

    • Mass

    • 2515.2

    • PI

    • 9.9

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +5

    • Boman Index

    • 8.35

    • Hydrophobicity

    • 0.833

    • Aliphatic Index

    • 141.67

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 8

DRAMP02770

DRAMP02770 chydropathy plot

Comments Information

    • Function

    • Shows moderate activity against E. coli and S. aureus (MIC<25 µM), slight activity against B. subtilis (MIC<50 µM), and no activity against L. garvieae, P. aeruginosa, C. albicans, and S. cerevisiae. Has no hemolytic nor cytolytic activity. Causes an IgE-independent histamine release.

    • Tissue specificity

    • Expressed by the venom gland.

Literature Information

  • ·Literature 1

    • Title

    • Molecular cloning and biological characterization of novel antimicrobial peptides, pilosulin 3 and pilosulin 4, from a species of the Australian ant genus Myrmecia.

    • Reference

    • Arch Biochem Biophys. 2004 Aug 15;428(2):170-178.

    • Author

    • Inagaki H, Akagi M, Imai HT, Taylor RW, Kubo T.