General Information

    • DRAMP ID

    • DRAMP02997

    • Peptide Name

    • Apidaecin-1B (Apidaecin IB; Insects, animals)

    • Source

    • Apis mellifera (Honeybee)

    • Family

    • Belongs to the apidaecin family

    • Gene

    • Not found

    • Sequence

    • GNNRPVYIPQPRPPHPRL

    • Sequence Length

    • 18

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.2676519] Gram-negative bacteria:
        Target OrganismActivity
        Agrobacterium tumefaciensMIC=0.2 μg/ml
        Erwinia salicisMIC=0.02 μg/ml
        Escherichia coliMIC=0.1 μg/ml
        Pseudomonas syringaeMIC=0.1 μg/ml
        Rhizobium melilotiMIC=0.02 μg/ml
        Salmonella NewportMIC=0.2 μg/ml
        Salmonella typhimuriumMIC=0.1 μg/ml
        Shigella flexneri (MIC=0.1 μg/ml);-
      • Gram-positive bacteria:
        Target OrganismActivity
        Corynebacterium insidiosumMIC=50 μg/ml
        Bacillus megateriumMIC=100 μg/ml
      • [Ref.2298215] Gram-negative bacteria:
        Target OrganismActivity
        Agrobacterium tumefaciens Gembloux AMIC≤1 µg/ml
        Erwinia salicis NCPPB 2530MIC≤1 µg/ml
        Escherichia coli NCTC 9001MIC≤1 µg/ml
        Escherichia coli K514MIC=25-50 µg/ml
        Pseudomonas syringae pv.tomato NCPPB 1105MIC≤1 µg/ml
        Xanthomonas campestris pv. vesicatoria LMG 905 (MIC≥100 µg/ml);-
      • Gram-positive bacteria:
        Target OrganismActivity
        Bacillus megateriurn QMB 1551MIC=25-50 µg/ml
        Bacillus alvei LMG 6922MIC=50-100 µg/ml
        Micrococcus lysodeikticus LMG 4050MIC=25-50 µg/ml

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02997 helical wheel diagram

    • PDB ID

    • 4E81 resolved by X-ray.

    • Predicted Structure

    • There is no predicted structure for DRAMP02997.

Physicochemical Information

    • Formula

    • C95H150N32O23

    • Absent Amino Acids

    • ACDEFKMSTW

    • Common Amino Acids

    • P

    • Mass

    • 2108.44

    • PI

    • 11.71

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 3

    • Net Charge

    • +4

    • Boman Index

    • -53.56

    • Hydrophobicity

    • -1.444

    • Aliphatic Index

    • 59.44

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 87.65

    • Polar Residues

    • 4

DRAMP02997

DRAMP02997 chydropathy plot

Comments Information

    • Function

    • Apidaecins have bactericidal activity; predominantly against Gram-negative bacteria. They seem to interfere with cell propagation.

Literature Information

  • ·Literature 1

    • Title

    • Apidaecins: antibacterial peptides from honeybees.

    • Reference

    • EMBO J. 1989 Aug;8(8):2387-2391.

    • Author

    • Casteels P, Ampe C, Jacobs F, Vaeck M, Tempst P.
  • ·Literature 2

    • Title

    • Isolation and characterization of abaecin, a major antibacterial response peptide in the honeybee (Apis mellifera).

    • Reference

    • Eur J Biochem. 1990 Jan 26;187(2):381-386.

    • Author

    • Casteels P, Ampe C, Riviere L, Van Damme J, Elicone C, Fleming M, Jacobs F, Tempst P.