General Information

    • DRAMP ID

    • DRAMP03007

    • Peptide Name

    • Osmin (Insects, animals)

    • Source

    • Osmia rufa (Red mason bee)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GFLSALKKYLPIVLKHV

    • Sequence Length

    • 17

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.19109988]Gram-positive bacterium: Micrococcus luteus (MIC=1.56-0.78 µM);
      • Gram-negative bacterium: Escherichia coli SBS363 (MIC=3.125-1.56 µM);
      • fungi: Fusarium oxysporum (MIC=0.78-0.39 µM).

    • Hemolytic Activity

      • [Ref.19109988]5.0 HU/mg hemolytic activity against human erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix (CD)

    • Structure Description

    • In absence of TFE, the spectrum shows a random coil type structure. When increasing the concentration of TFE from 0 to 20%, a transition into an α-helix structure is observed.

    • Helical Wheel Diagram

    • DRAMP03007 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03007.

Physicochemical Information

    • Formula

    • C95H156N22O20

    • Absent Amino Acids

    • CDEMNQRTW

    • Common Amino Acids

    • L

    • Mass

    • 1926.42

    • PI

    • 10

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +4

    • Boman Index

    • 13.56

    • Hydrophobicity

    • 0.806

    • Aliphatic Index

    • 154.71

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 93.13

    • Polar Residues

    • 3

DRAMP03007

Comments Information

    • Function

    • Synthetic peptide exhibits hemolytic activity to human erythrocytes and inhibits bacterial and fungal growth at micromolar concentrations. A synthetic peptide with a free C-terminus shows weaker hemolytic (5.0 HU/mg), antibacterial and antifungal activities.

    • Tissue specificity

    • Expressed by the venom gland.

    • PTM

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • Structural identification by mass spectrometry of a novel antimicrobial peptide from the venom of the solitary bee Osmia rufa (hymenoptera: megachilidae).

    • Reference

    • Toxicon. 2010 Jan;55(1):20-27.

    • Author

    • öcklin R, Favreau P, Thai R, Pflugfelder J, Bulet P, Mebs D.