General Information

    • DRAMP ID

    • DRAMP03420

    • Peptide Name

    • Neutrophil antibiotic peptide NP-2 (RatNP-2; Rodents, mammals, animals)

    • Source

    • Rattus norvegicus (Rat)

    • Family

    • Belongs to the alpha-defensin family

    • Gene

    • Defa

    • Sequence

    • VTCYCRSTRCGFRERLSGACGYRGRIYRLCCR

    • Sequence Length

    • 32

    • Protein Existence

    • Protein level

    • SMILES

    • CC[C@@H](C)[C@@H]1NC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](Cc2ccc(O)cc2)NC(=O)CNC(=O)[C@@H]2CSSC[C@@H]3NC(=O)[C@H](Cc4ccc(O)cc4)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)[C@@H](C)O)CSSC[C@@H](C(=O)N[C@@H](CCCNC(=N)N)C(=O)O)NC(=O)[C@H](CSSC[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(=N)N)NC3=O)C(=O)NCC(=O)N[C@@H](Cc3ccccc3)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](C)C(=O)N2)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](Cc2ccc(O)cc2)NC1=O

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-positive bacterium:
        Target OrganismActivity
        Staphylococcus aureus-
      • Gram-negative bacterium:
        Target OrganismActivity
        Salmonella typhimurium-

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys3 and Cys31,Cys5 and Cys20,Cys10 and Cys30.

    • Stereochemistry

    • L

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C155H254N56O42S6

    • Absent Amino Acids

    • DHKMNPQW

    • Common Amino Acids

    • R

    • Mass

    • 3766.43

    • PI

    • 9.69

    • Basic Residues

    • 8

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +7

    • Boman Index

    • -103.5

    • Hydrophobicity

    • -0.378

    • Aliphatic Index

    • 48.75

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 4845

    • Absorbance 280nm

    • 156.29

    • Polar Residues

    • 17

DRAMP03420

Comments Information

    • Active in vitro against S. aureus, fungi, Gram-positive and Gram-negative bacteria and to a lesser extent against an enveloped virus.

Literature Information

  • ·Literature 1

    • Title

    • Polymorphic expression of defensins in neutrophils from outbred rats.

    • Reference

    • Infect. Immun. 1990;58:3899-3902.

    • Author

    • Eisenhauer P.B, Harwig S.S.S.L, Szklarek D, Ganz T, Lehrer R.I.
  • ·Literature 2

    • Title

    • Rat neutrophil defensins. Precursor structures and expression during neutrophilic myelopoiesis.

    • Reference

    • J. Immunol. 1995;155:4476-4484.

    • Author

    • Yount N.Y, Wang MS.C, Yuan J, Banaiee N, Ouellette A.J, Selsted M.E.