General Information

    • DRAMP ID

    • DRAMP18322

    • Peptide Name

    • Hominicin (Bacteriocin)

    • Source

    • Staphylococcus hominis MBBL 2-9

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • ITPATPFTPAIITEITAAVIA

    • Sequence Length

    • 21

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • [Ref.20654578] Gram-positive bacteria: Staphylococcus aureus (MIC=0.06 µg/ml), MRSA (MIC=0.96 µg/ml), VISA (MIC=3.82 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • 1-DmIle(N,N-dimethyl Ile)-I; 2,5,8,15-DHB(dehydrated threonine)-T;21-Dmp(N2, N2-dimethyl-1,2-propanediamine)-A

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • NMR played a critical role in establishing the peptide primary structure. While the N-terminal residue is an N,N-dimethyl Ile (DmIle), the C-terminus is a N2, N2-dimethyl-1,2-propanediamine (Dmp). In addition, threonines are dehydrated into Dhb. In this sense, this peptide may be classified as a class I variant bacteriocin.

    • Helical Wheel Diagram

    • DRAMP18322 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP18322.

Physicochemical Information

    • Formula

    • C99H163N21O29

    • Absent Amino Acids

    • CDGHKLMNQRSWY

    • Common Amino Acids

    • AIT

    • Mass

    • 2111.51

    • PI

    • 4

    • Basic Residues

    • 0

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 12

    • Net Charge

    • -1

    • Boman Index

    • 2101

    • Hydrophobicity

    • 1.271

    • Aliphatic Index

    • 130.48

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 5

DRAMP18322

DRAMP18322 chydropathy plot

Comments Information

    • Function

    • Hominicin exhibited heat stability up to 121 degrees C for 15min and activity under both acidic and basic conditions (from pH 2.0 to 10.0). Hominicin exhibited distinct structural and attractive biochemical characteristics with a high bactericidal activity against MRSA and VISA, heat-tolerance and pH stability.

Literature Information

  • ·Literature 1

    • Title

    • Characterization and structure identification of an antimicrobial peptide, hominicin, produced by Staphylococcus hominis MBBL 2-9.

    • Reference

    • Biochem Biophys Res Commun. 2010 Aug 20;399(2):133-138.

    • Author

    • Kim PI, Sohng JK, Sung C, Joo HS, Kim EM, Yamaguchi T, Park D, Kim BG.