• • DRAMP ID

  • DRAMP20941

• • Peptide Name

  • [Pro3,DLeu9]TL(8) (Temporin L peptide derivative)

• • Source

  • Synthetic construct

• • Family

  • Derived from Temporin L

• • Gene

  • Not found

• • Sequence

  • FVPWFSKFLKRIL

• • Sequence Length

  • 13

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.28863356] Gram-positive bacteria:

      Target Organism	Activity
      Bacillus megaterium Bm11	MIC=3.125μM
      Staphylococcus aureus Cowan I	MIC=6.25μM
      Staphylococcus epidermidis ATCC 12228 (MIC=3.125μM);	-

    • Gram-negative bacteria:

      Target Organism	Activity
      Acinetobacter baumannii ATCC 19606	MIC=3.125μM
      Escherichia coli D21	MIC=12.5μM
      Pseudomonas syringae pv tabaci 1918	MIC=6.25μM
      Pseudomonas aeruginosa ATCC 2785 (MIC=12.5μM);	-

    • Yeasts: Candida albicans ATCC 10231 (MIC=1.56μM), Candida crusei ATCC 6258 (MIC=1.56μM)

• • Hemolytic Activity

  • • [Ref.28863356]49.30 ± 2.88% cytotoxic effect at 12.5 μM, 66.86 ± 2.81% cytotoxic effect at 25 μM, 96.79 ± 1.24% cytotoxic effect at 50 μM on HaCaT cells.

• • Cytotoxicity

  • • [Ref.28863356] The cytotoxic effect is 49.30±2.88%, 66.86±2.81% and 96.79±1.24% at peptide concentrations of 12.5, 25 and 50 μM

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • Mixed (D-Leu9)

• • Structure

  • Alpha helix

• • Structure Description

  • The analogues bearing a D residue showed a reduction in the percentage of helicity compared to the corresponding L analogues in the MMs

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP20941.

DRAMP20941

• • Function

  • Antimicrobial activity against the Gram-negative bacteria, Gram-positive bacteria and Fungi. The position 9 of [Pro3,DLeu9]TL(8) is D-Leucine, L-Lysine replace L-Proline at the position 10 on the peptide [Pro3,DLeu9]TL(2).

• ·Literature 1

• • Title

  • Glycine-replaced derivatives of [Pro3,DLeu9]TL, a temporin L analogue: Evaluation of antimicrobial, cytotoxic and hemolytic activities

• • Pubmed ID

  • 28863356

• • Reference

  • Eur J Med Chem. 2017 Oct 20;139:750-761.

• • Author

  • Merlino F, Carotenuto A, Casciaro B, MartorMerlino F, Carotenuto A, Casciaro B, Martora F, Loffredo MR, Di Grazia A, Yousif AM, Brancaccio D, Palomba L, Novellino E, Galdiero M, Iovene MR, Mangoni ML, Grieco P.