General Information
-
DRAMP ID
- DRAMP20956
-
Peptide Name
- AL32-P113
-
Source
- Synthetic construct
-
Family
- Derived from LL-37 and Hst-5
-
Gene
- Not found
-
Sequence
- ALGDFFRKSKEKIGKEFKRIVQRIKDFLRNLAKRHHGYKRKFHLEY
-
Sequence Length
- 46
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.29859288] Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=66.6 ± 28.8 μg/mL);
- Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=41.6 ± 14.4 μM), Extended-spectrum beta-lactamase producing Escherichia coli (MIC=41.6 ± 14.4 μg/mL), New Delhi metallo-bata-lactamase-1 producing Acinetobacter baumannii (MIC=20.8 ± 7.2 μg/mL);
- Fungi: Candida albicans ATCC 90028 (MIC=166.6 ± 57.7 μg/mL)
-
Hemolytic Activity
-
- [Ref.29859288] Hemolysis 10.4 ± 2.2% at 500 μg/ml against human red blood cells
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Alpha helix
-
Structure Description
- The peptide adopts a conformational structure with 81 % alpha helix.
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP20956.
Physicochemical Information
-
Formula
- C265H421N81O62
Absent Amino Acids
- CMPTW
Common Amino Acids
- K
Mass
- 5733.76
PI
- 10.6
Basic Residues
- 18
Acidic Residues
- 5
Hydrophobic Residues
- 15
Net Charge
- +13
-
Boman Index
- -14736
Hydrophobicity
- -1.094
Aliphatic Index
- 70
Half Life
-
- Mammalian:4.4 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 2980
Absorbance 280nm
- 66.22
Polar Residues
- 7
DRAMP20956
![DRAMP20956 chydropathy plot](http://dramp.cpu-bioinfor.org/tmp/all_info_png/pepwindow_DRAMP20956.1.png)
Comments Information
Function
- Antibacterial activity against the Gram-positive and Gram-negative bacteria. Antifungal activity.
Literature Information
- ·Literature 1
-
Title
- Expression in Escherichia coli of novel recombinant hybrid antimicrobial peptide AL32-P113 with enhanced antimicrobial activity in vitro.
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Pubmed ID
- 29859288
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Reference
- Gene. 2018 May 30. pii: S0378-1119(18)30617-6.
-
Author
- Wanmakok M, Orrapin S, Intorasoot A, Intorasoot S.