• • DRAMP ID

  • DRAMP21066

• • Peptide Name

  • TP1[R17A] (Derived from TP1)

• • Source

  • Synthetic construct

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • KWCFRVCYRGICYRRCA

• • Sequence Length

  • 17

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.28960954] Gram-positive bacteria : Bacillus subtilis ATCC 6051(MIC=0.015 μg/ml), Staphylococcus aureus ATCC 43300 (MRSA)(MIC=0.5 μg/ml);
    • Gram-negative bacteria : Escherichia coli ATCC 25922(MIC=0.015 μg/ml), Klebsiella pneumoniae ATCC 13883(MIC=0.125 μg/ml), Klebsiella pneumoniae ATCC 700603 (MDR)(MIC=0.25 μg/ml), Klebsiella pneumoniae BAA 2146 (NDM-1 pos)(MIC=0.25 μg/ml), Klebsiella pneumoniae clinical isolate 100650661 : 1 (XDR;PmxR)(MIC=4 μg/ml), Acinetobacter baumannii ATCC 19606(MIC=0.125 μg/ml), Acinetobacter baumannii clinical isolate 100734512 : 2 (XDR;PmxR)(MIC=0.125 μg/ml), Pseudomonas aeruginosa ATCC 27853(MIC=0.25 μg/ml), Pseudomonas aeruginosa FADDIFA070 (PmxR)(MIC=0.5 μg/ml);
    • Fungi : Candida albicans ATCC 90028(MIC=4 μg/ml), Cryptococcus neoformans ATCC 208821(MIC=0.25 μg/ml)

• • Hemolytic Activity

  • • [Ref.28960954] MHC10=0.45 μg/ml against human red blood cells

• • Cytotoxicity

  • • [Ref.28960954] The peptide did not show cytotoxicity against HEK293 cells at the highest tested concentration, 100 μg/ml.

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bonds between Cys3 and Cys16, Cys7 and Cys12.

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• Please click DRAMP21066_predicted_structure.pdb to download.

DRAMP21066

• • Function

  • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans, Cryptococcus neoformans

• ·Literature 1

• • Title

  • Structure-Activity and -Toxicity Relationships of the Antimicrobial Peptide Tachyplesin-17

• • Pubmed ID

  • 28960954

• • Reference

  • ACS Infect Dis. 2017 Dec 8;3(12):917-926. doi: 10.1021/acsinfecdis.7b00123.

• • Author

  • Ingrid A. Edwards , Alysha G. Elliott , Angela M. Kavanagh , Mark A. T. Blaskovich , and Matthew A. Cooper