General Information

    • DRAMP ID

    • DRAMP21317

    • Peptide Name

    • 3W-4 (Derived from PMAP-36)

    • Source

    • synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RWLRWKTRWRLK

    • Sequence Length

    • 12

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.31276398] Gram-positive bacteria:Staphylococcus aureus 29213 (MIC=4 μM), Staphylococcus aureus 25923 (MIC=16 μM), Staphylococcus epidermidis 12228 (MIC=16 μM);
      • Gram-negative bacteria: Escherichia coli 25922 (MIC=2 μM), Escherichia coli 1005 (MIC=4 μM), Staphylococcus typhi (MIC=8 μM)

    • Hemolytic Activity

      • [Ref.31276398] MHC>128μM. The minimum hemolytic concentration (MHC) is measured as the lowest concentration of the peptides that caused 5% hemolysis of human red blood cells (hRBCs).

    • Cytotoxicity

      • [Ref.31276398] The cell viability of the murine macrophage cell line RAW264.7 induced by 3W-4 is 100.2%, 92.2%, 96.2%, 89.0%, 94.1%, 97.4%, 94.3% and 88.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 and 128 μM

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • α-helix in 50%TFE

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21317 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C85H133N29O14

    • Absent Amino Acids

    • ACDEFGHIMNPQSVY

    • Common Amino Acids

    • R

    • Mass

    • 1785.18

    • PI

    • 12.48

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +6

    • Boman Index

    • -5652

    • Hydrophobicity

    • -1.8

    • Aliphatic Index

    • 65

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 16500

    • Absorbance 280nm

    • 1500

    • Polar Residues

    • 1

DRAMP21317

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Design of Trp-rich dodecapeptides with broad-spectrum antimicrobial potency and membrane-disruptive mechanism

    • Reference

    • J Med Chem. 2019 Aug 8;62(15):6941-6957. doi: 10.1021/acs.jmedchem.9b00295.

    • Author

    • Lyu Y, Chen T, Shang L, Yang Y, Li Z, Zhu J, Shan A.