• • DRAMP ID

  • DRAMP29140

• • Peptide Name

  • Pxt‐2

• • Source

  • Xenopus tropicalis

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • FIGALLRPALKLLA

• • Sequence Length

  • 14

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Not found

• • Biological Activity

  • Antimicrobial, Antibacterial,Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.25312021]Gram-negative bacteria:Escherichia coli DH5α(MIC=50 µg/ml);
    • Gram-positive bacteria:Staphylococcus aureus JCM 2151(MIC=9.7 µg/ml).
    • [Ref.26802742]Gram-negative bacteria:Escherichia coli NBRC 14237(MIC<12.5 µM);Pseudomonas aeruginosa NBRC 12582(MIC<50 µM);
    • Gram-positive bacteria:Staphylococcus aureus NBRC 12732(MIC<12.5 µM);Micrococcus luteus NBRC 12708(MIC<25 µM);
    • Fungi:Saccharomyces cerevisiae NBRC 10217(ND);Candida albicans NBRC 1594(ND).

• • Hemolytic Activity

  • • [Ref.25312021]0.4% Hemolysis against Rat erythrocytes at 20 µM.
    • [Ref.26802742]2% Hemolysis against Rat erythrocytes at 50 µM.

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Alpha helix,random coil

• • Structure Description

  • 3.8%,4.0%,4.9% α-helix were mearsured by CD spectra at 10 °C, 25 °C and 37 °C in 10 mm Tris-HCl (pH 7.2).

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP29140.

DRAMP29140

• • Function

  • showed growth inhibitory effects on both Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).

• ·Literature 1

• • Title

  • Identification of novel peptides from amphibian (Xenopus tropicalis) skin by direct tissue MALDI-MS analysis.

• • Pubmed ID

  • 25312021

• • Reference

  • FEBS J. 2015 Jan;282(1):102-13.

• • Author

  • Shigeri Y, Yasuda A, Hagihara Y, Nishi K, Watanabe K, Imura T, Inagaki H, Haramoto Y, Ito Y, Asashima M.

• ·Literature 2

• • Title

  • Physicochemical and biological characterizations of Pxt peptides from amphibian (Xenopus tropicalis) skin.

• • Pubmed ID

  • 26802742

• • Reference

  • J Biochem. 2016 Jun;159(6):619-29.

• • Author

  • Shigeri Y, Horie M, Yoshida T, Hagihara Y, Imura T, Inagaki H, Haramoto Y, Ito Y, Asashima M.