• • DRAMP ID

  • DRAMP29141

• • Peptide Name

  • Pxt‐3

• • Source

  • Xenopus tropicalis

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • GLKEVAHSAKKFAKGFISGLTGS

• • Sequence Length

  • 23

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Not found

• • Biological Activity

  • Antimicrobial, Antibacterial,Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.25312021]Gram-negative bacteria:Escherichia coli DH5α(Not active up to 150 µg/ml);
    • Gram-positive bacteria:Staphylococcus aureus JCM 2151(Not active up to 150 µg/ml).
    • [Ref.23935531]Gram-negative bacteria:Escherichia coli ATCC 25922(MIC=64 µm);Pseudomonas aeruginosa ATCC 15692(MIC >256 µM);
    • Gram-positive bacteria:Staphylococcus aureus ATCC 25923(MIC >256 µM);Micrococcus luteus(MIC=32 µM);
    • Fungi:Saccharomyces cerevisiae(MIC=128 µM);
    • Parasite:Trypanosoma brucei(LC95=8->256 µM).

• • Hemolytic Activity

  • • [Ref.25312021]0.2% Hemolysis against Rat erythrocytes at 20 µM.
    • [Ref.23935531] Hemolysis against Mouse erythrocytes (HC50>256 µm).

• • Cytotoxicity

  • • [Ref.23935531]Cytotoxicity against T-lymphocytes(IC50>256 µM).

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Alpha helix,random coil

• • Structure Description

  • 0.5%,1.2%,2.0% α-helix were mearsured by CD spectra at 10 °C, 25 °C and 37 °C in 10 mm Tris-HCl (pH 7.2).

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP29141.

DRAMP29141

• • Function

  • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria.

• ·Literature 1

• • Title

  • Identification of novel peptides from amphibian (Xenopus tropicalis) skin by direct tissue MALDI-MS analysis.

• • Pubmed ID

  • 25312021

• • Reference

  • FEBS J. 2015 Jan;282(1):102-13.

• • Author

  • Shigeri Y, Yasuda A, Hagihara Y, Nishi K, Watanabe K, Imura T, Inagaki H, Haramoto Y, Ito Y, Asashima M.

• ·Literature 2

• • Title

  • Origin and functional diversification of an amphibian defense peptide arsenal.

• • Pubmed ID

  • 23935531

• • Reference

  • PLoS Genet. 2013;9(8):e1003662.

• • Author

  • Roelants K, Fry BG, Ye L, Stijlemans B, Brys L, Kok P, Clynen E, Schoofs L, Cornelis P, Bossuyt F.