General Information

    • DRAMP ID

    • DRAMP29149

    • Peptide Name

    • Reverse Pxt-2

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • ALLKLAPRLLAGIF

    • Sequence Length

    • 14

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial,Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.26802742]Gram-negative bacteria:Escherichia coli NBRC 14237(MIC<12.5 µM);Pseudomonas aeruginosa NBRC 12582(MIC<50 µM);
      • Gram-positive bacteria:Staphylococcus aureus NBRC 12732(MIC<12.5 µM);Micrococcus luteus NBRC 12708(MIC<50 µM);
      • Fungi:Candida albicans NBRC 1594(ND).

    • Hemolytic Activity

      • [Ref.26802742]1.5% Hemolysis against Rat erythrocytes at 50 µM.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • liposomes

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix,random coil

    • Structure Description

    • 3.6%,4.7%,7.3% α-helix were mearsured by CD spectra at 10 °C, 25 °C and 37 °C in 10 mm Tris-HCl (pH 7.2);28.6%,26.8%,22.8% α-helix were mearsured by CD spectra at 10 °C, 25 °C and 37 °C in 50% TFE.

    • Helical Wheel Diagram

    • DRAMP29149 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29149.

Physicochemical Information

    • Formula

    • C73H126N18O15

    • Absent Amino Acids

    • CDEHMNQSTVWY

    • Common Amino Acids

    • L

    • Mass

    • 1495.91

    • PI

    • 11

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +2

    • Boman Index

    • 1840

    • Hydrophobicity

    • 1.521

    • Aliphatic Index

    • 188.57

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 1

DRAMP29149

Comments Information

    • Induced a significant and dose-dependent histamine release.

Literature Information

  • ·Literature 1

    • Title

    • Physicochemical and biological characterizations of Pxt peptides from amphibian (Xenopus tropicalis) skin.

    • Reference

    • J Biochem. 2016 Jun;159(6):619-29.

    • Author

    • Shigeri Y, Horie M, Yoshida T, Hagihara Y, Imura T, Inagaki H, Haramoto Y, Ito Y, Asashima M.