• • DRAMP ID

  • DRAMP29157

• • Peptide Name

  • EK1C4

• • Source

  • Synthetic construct

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • SLDQINVTFLDLEYEMKKLEEAIKKLEESYIDLKELGSGSG

• • Sequence Length

  • 41

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Not found

• • SMILES

  • Not available

• • Biological Activity

  • Antimicrobial, Antiviral(SARS-CoV-2)

• • Target Organism

  • • [Ref.35087243]Virus:
    • SARS-CoV-2 Omicron:

      Target Organism	Activity
      inhibition of cell-cell fusion in Calu-3 cells(IC50=3.32 nM);inhibition of cell-cell infusion in Caco2 cells(IC50=0.88 nM);inhibition of infection(Pseudovirus)(IC50=8.63 nM);inhibition of infection(Authentic)	IC50=85.38 nM

    • SARS-CoV-2 Delta:

      Target Organism	Activity
      inhibition of cell-cell fusion(IC50=4.04 nM);inhibition of infection(Pseudovirus)	IC50=9.83 nM

    • SARS-CoV-2 D614G:

      Target Organism	Activity
      inhibition of cell-cell fusion(IC50=2.57 nM);inhibition of infection(Pseudovirus)	IC50=5.58 nM

    • [Ref.32231345]Virus:
    • SARS-CoV:

      Target Organism	Activity
      ihibition of cell-cell fusion in Huh-7 cells	IC50=4.3 nM
      inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells	IC50=11.7 nM

    • MERS-CoV:

      Target Organism	Activity
      ihibition of cell-cell fusion in Huh-7 cells	IC50=2.5 nM
      inhibition of Pseudovirus (PsV) infection in Huh-7 cells	IC50=11.1 nM
      inhibit the replication of MERS-CoV in VERO-E6 cells	IC50=4.2 nM

    • HCoV-OC43: ihibition of cell-cell fusion in Huh-7 cells(IC50=7.7 nM),inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells(IC50=37.7 nM),inhibit the replication of HCoV-OC43 in RD cells(IC50=24.8 nM);
    • HCoV-229E: ihibition of cell-cell fusion in Huh-7 cells(IC50=5.2 nM),inhibition of Pseudovirus (PsV) infection in Huh-7 cells(IC50=12.4 nM),inhibit the replication of HCoV-229E in Huh-7 cells(IC50=101.5 nM);
    • HCoV-NL63: ihibition of cell-cell fusion in Huh-7 cells(IC50=21.4 nM),inhibition of Pseudovirus (PsV) infection in Huh-7 cells(IC50=76.6 nM),inhibit the replication of HCoV-NL63 in LLC-MK2 cells(IC50=187.6 nM);
    • CoV-WIV1: ihibition of cell-cell fusion in Huh-7 cells(IC50=4.5 nM),inhibition of Pseudovirus (PsV) infection in Huh-7 cells(IC50=30.8 nM);
    • CoV-Rs3367: ihibition of cell-cell fusion in Huh-7 cells(IC50=8.1 nM),inhibition of Pseudovirus (PsV) infection in Huh-7 cells(IC50=66.9 nM);
    • CoV-SHC014: ihibition of cell-cell fusion in Huh-7 cells(IC50=4.3 nM);
    • SARS-CoV-2:

      Target Organism	Activity
      ihibition of cell-cell fusion in 293T/ACE2 cells	IC50=1.3 nM
      inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells	IC50=15.8 nM
      inhibit the replication of MERS-CoV in VERO-E6 cells	IC50=2468 nM

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • [Ref.32231345]<10% cytotoxicity against VERO-E6 cells, RD cells, LLC-MK2 cells, Huh-7 cells up to 10000 nM.

• • Binding Target

  • liposomes

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • PEG4-Chol

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • PDB ID

  • None

• Predicted Structure

• Please click DRAMP29157_predicted_structure.pdb to download.

DRAMP29157

• • Mechanism of action

  • The peptide acted as a fusion inhibitor which against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection.

• ·Literature 1

• • Title

  • Peptide-based pan-CoV fusion inhibitors maintain high potency against SARS-CoV-2 Omicron variant.

• • Pubmed ID

  • 35087243

• • Reference

  • Cell Res. 2022 Apr;32(4):404-406.

• • Author

  • Xia S, Chan JF, Wang L, Jiao F, Chik KK, Chu H, Lan Q, Xu W, Wang Q, Wang C, Yuen KY, Lu L, Jiang S.

• ·Literature 2

• • Title

  • Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion.

• • Pubmed ID

  • 32231345

• • Reference

  • Cell Res. 2020 Apr;30(4):343-355.

• • Author

  • Xia S, Liu M, Wang C, Xu W, Lan Q, Feng S, Qi F, Bao L, Du L, Liu S, Qin C, Sun F, Shi Z, Zhu Y, Jiang S, Lu L.