General Information

    • DRAMP ID

    • DRAMP29179

    • Peptide Name

    • IPB01(SARS-CoV-S (1151-1185),SR9, SARS-CoV-2-S (1169-1203))

    • Source

    • Synthetic construct

    • Family

    • Belongs to the betacoronaviruses spike protein family.

    • Gene

    • S

    • Sequence

    • ISGINASVVNIQKEIDRLNEVAKNLNESLIDLQEL

    • Sequence Length

    • 35

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antiviral(SARS-CoV-2)

    • Target Organism

      • [Ref.32376627]Virus:
      • SARS-CoV-2:ihibition of cell-cell fusion in 293T/ACE2 cells(IC50=0.022±0.005 µM),inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells(IC50=33.74±11.827 µM);
      • SARS-CoV:inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells(IC50>50 µM);
      • Vesicular Stomatitis Virus (VSV):inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells(IC50>50 µM).
      • [Ref.17942557]Virus:SARS-CoV:Inhibition of virus entry in VERO-E6 cells(EC50=0.005 µM).
      • [Ref.18442051]Virus:SARS-CoV: inhibition of PsV entry in Vero-E6 cells(EC50=0.34 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • liposomes

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP29179 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29179.

Physicochemical Information

    • Formula

    • C168H287N47O58

    • Absent Amino Acids

    • CFHMPTWY

    • Common Amino Acids

    • ILN

    • Mass

    • 3893.41

    • PI

    • 4.36

    • Basic Residues

    • 3

    • Acidic Residues

    • 6

    • Hydrophobic Residues

    • 15

    • Net Charge

    • -3

    • Boman Index

    • -5930

    • Hydrophobicity

    • -0.083

    • Aliphatic Index

    • 142

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 9

DRAMP29179

DRAMP29179 chydropathy plot

Comments Information

    • Mechanism of action

    • The peptide was designed based on HR2 sequence lipopeptide fusion inhibitor,which showed highly potent activities in inhibiting SARS-CoV-2 S protein-mediated cell-cell fusion and pseudovirus transduction. It can also inhibit the SARS-CoV pseudovirus efficiently.

Literature Information

  • ·Literature 1

    • Title

    • Heptad repeat-derived peptides block protease-mediated direct entry from the cell surface of severe acute respiratory syndrome coronavirus but not entry via the endosomal pathway.

    • Reference

    • J Virol. 2008 Jan;82(1):588-92.

    • Author

    • Ujike M, Nishikawa H, Otaka A, Yamamoto N, Yamamoto N, Matsuoka M, Kodama E, Fujii N, Taguchi F.
  • ·Literature 2

    • Title

    • Fusion core structure of the severe acute respiratory syndrome coronavirus (SARS-CoV): in search of potent SARS-CoV entry inhibitors.

    • Reference

    • J Cell Biochem. 2008 Aug 15;104(6):2335-47.

    • Author

    • Chu LH, Chan SH, Tsai SN, Wang Y, Cheng CH, Wong KB, Waye MM, Ngai SM.
  • ·Literature 3

    • Title

    • Design of Potent Membrane Fusion Inhibitors against SARS-CoV-2, an Emerging Coronavirus with High Fusogenic Activity.

    • Reference

    • J Virol. 2020 Jul 1;94(14):e00635-20.

    • Author

    • Zhu Y, Yu D, Yan H, Chong H, He Y.