General Information
-
DRAMP ID
- DRAMP00002
-
Peptide Name
- Entianin (Bacteriocin)
-
Source
- Bacillus subtilis subsp. spizizenii DSM 15029(T) (Gram-positive bacteria)
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Family
- Belongs to the lantibiotic family (Class I bacteriocin)
-
Gene
- Not found
-
Sequence
- WKSESVCTPGCVTGLLQTCFLQTITCNCKISK
-
Sequence Length
- 32
-
UniProt Entry
- No entry found
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial
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Target Organism
-
- [Ref.21239550] Gram-positive bacteria: Staphylococcus aureus ATCC 29213 (MIC = 4-8 μg/ml),Staphylococcus aureus ATCC 43300 (MRSA)(MIC = 8 μg/ml), Enterococcus faecalis ATCC 29212(MIC = 16μg/ml), Micrococcus luteus ATCC 9341(MIC = 4-8μg/ml), Enterococcus faecalis ATCC 51299 (VRE) (MIC = 8-16 μg/ml).
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Succinylation
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- There are two dehydroalanines (S5; S31),one dehydrobutyrine (T18),one lanthionine (S3-C7) and four β-methyllanthionines (T8-C11; T13-C19; T23-C26; T25-C28).
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Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP00002.
Physicochemical Information
-
Formula
- C150H247N39O46S5
Absent Amino Acids
- ADHMRY
Common Amino Acids
- CT
Mass
- 3493.14
PI
- 8.42
Basic Residues
- 3
Acidic Residues
- 1
Hydrophobic Residues
- 9
Net Charge
- +2
-
Boman Index
- -17.96
Hydrophobicity
- 0.288
Aliphatic Index
- 79.06
Half Life
-
- Mammalian:2.8 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5750
Absorbance 280nm
- 185.48
Polar Residues
- 16
DRAMP00002
Comments Information
Function
- Entianin is very active against several Gram-positive pathogens, such as Staphylococcus aureus and Enterococcus faecalis.
Literature Information
- ·Literature 1
-
Title
- Entianin, a Novel Subtilin-Like Lantibiotic from Bacillus subtilis DSM 15029T with High Antimicrobial Activity.
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Pubmed ID
- 21239550
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Reference
- Appl Environ Microbiol. 2011 Mar;77(5):1698-1707.
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Author
- Fuchs SW, Jaskolla TW, Bochmann S, Kötter P, Wichelhaus T, Karas M, Stein T, Entian KD.