• • DRAMP ID

  • DRAMP00017

• • Peptide Name

  • Microbisporicin A1 (Bacteriocin)

• • Source

  • Microbispora corallina (Gram-positive bacteria)

• • Family

  • Belongs to the lantibiotic family (Class I bacteriocin)

• • Gene

  • Not found

• • Sequence

  • VTSWSLCTPGCTSPGGGSNCSFCC

• • Sequence Length

  • 24

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial

• • Target Organism

  • • Human pathogens:

      Human Pathogen	Activity
      L100 Staphylococcus aureus ATCC6538P	MIC≤0.13 μg/ml
      L819 Staphylococcus aureus Smith ATCC19636	MIC≤0.13 μg/ml
      L1400 S. aureus MRSA	MIC≤0.13 μg/ml
      L613 S. aureus MRSA	MIC≤0.13 μg/ml
      L3798 S. aureus VISA	MIC=2 μg/ml
      L3798 Staphylococcus epidermidis ATCC12228	MIC≤0.13 μg/ml
      L1729 Staphylococcus haemolyticus met-r	MIC=8 μg/ml
      L49 Streptococcus pyogenes	MIC≤0.13 μg/ml
      L44 Streptococcus pneumoniae	MIC≤0.13 μg/ml
      L559 Enterococcus faecalis	MIC=1 μg/ml
      L560 Enterococcus faecalis Van A	MIC=0.5 μg/ml
      LA533 Enterococcus faecalis Van A	MIC=1 μg/ml
      L568 Enterococcus faecium	MIC=2 μg/ml
      L569 Enterococcus faecium Van A	MIC=1 μg/ml
      LB518 E. faecium Van A	MIC=2 μg/ml
      L884 Lactobacillus garviae	MIC=1 μg/ml
      L148 Lactobacillus delbrueckii ATCC04797	MIC=4 μg/ml
      L3607 Clostridium perfringens ATCC13124	MIC≤0.125 μg/ml
      L4018 Clostridium difficile	MIC≤0.125 μg/ml
      L4043 Clostridium butyricum	MIC≤0.125 μg/ml
      Propionibacterium granulosum ATCC25564	MIC=0.03 μg/ml
      L1329 Propionibacterium acnes	MIC=0.5 μg/ml
      Propionibacterium limphophylum ATCC27250	MIC=0.015 μg/ml
      L970 Haemophilus influenzae ATCC19418	MIC=32 μg/ml
      L76 Moraxella catarrhalis ATCC8176	MIC=0.25 μg/ml
      L1613 Neisseria meningitidis ATCC13090V	MIC=0.5 μg/ml
      L997 Neisseria gonorrhoeae	MIC=0.25 μg/ml

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation and Cyclization

• • Nonterminal Modifications and Unusual Amino Acids

  • ①There are five thioether intramolecular bridges which link Ala3 and Ala7, Ala8 and Ala11, Ala13 and Ala20, Ala18 and Ala23, respectively. ②The residue at position 2 is (Z)-2,3-didehydrobutyrine (Dhb). ③The residue at position 4 is chloro-tryptophan (ClTrp). ④The residue at position 5 is 2,3-didehydroalanine. ⑤The residue at position 8 is 2-Aminobutyric acid (Abu). ⑥The residue at position 14 is bis-hydroxylated proline.

• • Stereochemistry

  • L

• • Structure

  • Rich

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP00017.

DRAMP00017

• • Function

  • Microbisporicins A1 displayed similar antibacterial activity by selectively blocking peptidoglycan biosynthesis, leading to cytoplasmic accumulation of the UDP-linked precursor.

• • PTM

  • Microbisporicin A1 contains five ether rings

• ·Literature 1

• • Title

  • Determining the structure and mode of action of microbisporicin, a potent lantibiotic active against multiresistant pathogens.

• • Pubmed ID

  • 18215770

• • Reference

  • Chem Biol. 2008 Jan;15(1):22-31.

• • Author

  • Castiglione F, Lazzarini A, Carrano L, Corti E, Ciciliato I, Gastaldo L, Candiani P, Losi D, Marinelli F, Selva E, Parenti F.