• DRAMP ID

    • DRAMP00032
    • Peptide Name

    • Ruminococcin A (RumA; Bacteriocin)
    • Source

    • Ruminococcus gnavus & Ruminococcus hansenii (Gram-positive bacteria)
    • Family

    • Belongs to the type A lantibiotic family (Class I bacteriocin)
    • Gene

    • rumA1 AND rumA2 AND
    • Sequence

    • GNGVLKTISHECNMNTWQFLFTCC
    • Sequence Length

    • 24
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial
    • Target Organism

      • Gram-positive bacteria: Bifidobacterium longum (strain: ATCC 15707) (MIC=75 µg/ml), Bacillus cereus (strain: Z4222, TZ415) (MIC=75 µg/ml), Eubacterium contortum (strain: ATCC 25540) (MIC=32.5 µg/ml), Ruminococcus obeum (strain: ATCC 29174) (MIC=32.5 µg/ml), R. gnavus (strain: ATCC 29149) (MIC=75 µg/ml), Clostridium perfringens (strain: CpA) (MIC=75 µg/ml), Clostridium difficile serogroup A (strain: B-1) (MIC=75 µg/ml), C. difficile serogroup C (strain: C253) (MIC=75 µg/ml), C. difficile serogroup S3 (strain: 79685) (MIC=75 µg/ml), C. botulinum type A (strain: CIP 38) (MIC=75 µg/ml), C. botulinum type B (strain: NCTC 7273) (MIC=75 µg/ml), C. botulinum type E (strain: NCTC 8266) (MIC=75 µg/ml), C. bifermentans (strain: NCTC 506) (MIC=75 µg/ml).
    • Hemolytic activity

    • Unknown
    • Binding Traget

    • Cell membrane
    • Structure

    • Unknow
    • Structure Description

    • Unknow
    • PDB ID

    • Unknow
    • DRAMP00032 helical wheel diagram
    • Formula

    • C119H180N32O35S4
    • Absent Amino Acids

    • ADPRY
    • Common Amino Acids

    • CNT
    • Mass

    • 3157.61
    • PI

    • 7.18
    • Basic Residues

    • 2
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 7
    • Boman Index

    • -18.43
    • Hydrophobicity

    • 8.75
    • Aliphatic Index

    • 60.83
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 5625
    • Absorbance 280nm

    • 244.57
    • Polar Residues

    • 12

Amino Acid Distribution

DRAMP00032 chydropathy plot
    • Function

    • Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. Ruminococcin A is a broad spectrum bacteriocin exhibiting activity against a wide range of pathogenic clostridia and B.longum.
    • Developmental stage

    • Highest expression in mid to late logarithmic growth phase, after which expression decreases slowly before leveling off in the stationary growth phase.
    • Induction

    • By trypsin.
    • PTM

    • There are one didehydrobutyrine (Dhb) at T16, one lanthionine (S9-23) and two Beta-methyllanthionines (T7-C12; T22-C24).
  • Literature 1
    • Reference

    • Appl Environ Microbiol. 2001 Sep;67(9):4111-4118.
    • Author

    • Dabard J, Bridonneau C, Phillipe C, Anglade P, Molle D, Nardi M, Ladiré M, Girardin H, Marcille F, Gomez A, Fons M.
    • Title

    • Ruminococcin A, a new lantibiotic produced by a Ruminococcus gnavus strain isolated from human feces.
  • Literature 2
    • Reference

    • J Bacteriol. 2002 Jan;184(1):18-28.
    • Author

    • Gomez A, Ladir© M, Marcille F, Fons M.
    • Title

    • Trypsin mediates growth phase-dependent transcriptional tegulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota.
  • Literature 3
    • Reference

    • Appl Environ Microbiol. 2002 Jul;68(7):3424-3431.
    • Author

    • Marcille F, Gomez A, Joubert P, Ladir© M, Veau G, Clara A, Gavini F, Willems A, Fons M.
    • Title

    • Distribution of genes encoding the trypsin-dependent lantibiotic ruminococcin A among bacteria isolated from human fecal microbiota.