• DRAMP ID

    • DRAMP00032
    • Peptide Name

    • Ruminococcin A (RumA; Bacteriocin)
    • Source

    • Ruminococcus gnavus & Ruminococcus hansenii (Gram-positive bacteria)
    • Family

    • Belongs to the type A lantibiotic family (Class I bacteriocin)
    • Gene

    • rumA1 AND rumA2 AND
    • Sequence

    • GNGVLKTISHECNMNTWQFLFTCC
    • Sequence Length

    • 24
    • Protein Existence

    • Protein level
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+
    • Target Organism

      • Gram-positive bacteria: Bifidobacterium longum (strain: ATCC 15707) (MIC=75 µg/ml), Bacillus cereus (strain: Z4222, TZ415) (MIC=75 µg/ml), Eubacterium contortum (strain: ATCC 25540) (MIC=32.5 µg/ml), Ruminococcus obeum (strain: ATCC 29174) (MIC=32.5 µg/ml), R. gnavus (strain: ATCC 29149) (MIC=75 µg/ml), Clostridium perfringens (strain: CpA) (MIC=75 µg/ml), Clostridium difficile serogroup A (strain: B-1) (MIC=75 µg/ml), C. difficile serogroup C (strain: C253) (MIC=75 µg/ml), C. difficile serogroup S3 (strain: 79685) (MIC=75 µg/ml), C. botulinum type A (strain: CIP 38) (MIC=75 µg/ml), C. botulinum type B (strain: NCTC 7273) (MIC=75 µg/ml), C. botulinum type E (strain: NCTC 8266) (MIC=75 µg/ml), C. bifermentans (strain: NCTC 506) (MIC=75 µg/ml).
    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Binding Target

    • Cell membrane
    • Linear/Cyclic

    • Cyclic (very possibly)
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Cyclization (possibly)
    • Nonterminal Modifications and Unusual Amino Acids

    • ① It is highly probable that (i) Ser9 and the putative Cys23 and (ii) the putative His22 and Thr24 are associated in a Lanthionine (Lan) and a 3-methyllanthionine (Me-Lan) residue, respectively. ②The residues at position 7 and 16 are (Z)-2,3-didehydrobutyrine (Dhb).
    • Stereochemistry

    • L
    • Structure

    • Not found
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP00032 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP00032.
    • Formula

    • C119H180N32O35S4
    • Absent Amino Acids

    • ADPRY
    • Common Amino Acids

    • CNT
    • Mass

    • 2747.17
    • PI

    • 6.72
    • Basic Residues

    • 2
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 7
    • Net Charge

    • +1
    • Boman Index

    • -18.43
    • Hydrophobicity

    • 0.088
    • Aliphatic Index

    • 60.83
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 5625
    • Absorbance 280nm

    • 244.57
    • Polar Residues

    • 12

DRAMP00032

DRAMP00032 chydropathy plot
    • Function

    • Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. Ruminococcin A is a broad spectrum bacteriocin exhibiting activity against a wide range of pathogenic clostridia and B.longum.
    • Developmental stage

    • Highest expression in mid to late logarithmic growth phase, after which expression decreases slowly before leveling off in the stationary growth phase.
    • Induction

    • By trypsin.
    • PTM

    • There are one didehydrobutyrine (Dhb) at T16, one lanthionine (S9-23) and two Beta-methyllanthionines (T7-C12; T22-C24).
  • ·Literature 1
    • Title

    • Ruminococcin A, a new lantibiotic produced by a Ruminococcus gnavus strain isolated from human feces.
    • Reference

    • Appl Environ Microbiol. 2001 Sep;67(9):4111-4118.
    • Author

    • Dabard J, Bridonneau C, Phillipe C, Anglade P, Molle D, Nardi M, Ladiré M, Girardin H, Marcille F, Gomez A, Fons M.
  • ·Literature 2
    • Title

    • Trypsin mediates growth phase-dependent transcriptional tegulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota.
    • Reference

    • J Bacteriol. 2002 Jan;184(1):18-28.
    • Author

    • Gomez A, Ladiré M, Marcille F, Fons M.
  • ·Literature 3
    • Title

    • Distribution of genes encoding the trypsin-dependent lantibiotic ruminococcin A among bacteria isolated from human fecal microbiota.
    • Reference

    • Appl Environ Microbiol. 2002 Jul;68(7):3424-3431.
    • Author

    • Marcille F, Gomez A, Joubert P, Ladiré M, Veau G, Clara A, Gavini F, Willems A, Fons M.