General Information

    • DRAMP ID

    • DRAMP00036

    • Peptide Name

    • Nisin A (Bacteriocin; Preclinical)

    • Source

    • Lactococcus lactis subsp. lactis (Streptococcus lactis) (Gram-positive bacteria)

    • Family

    • Belongs to the type A lantibiotic family (Class I bacteriocin)

    • Gene

    • spaN

    • Sequence

    • ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK

    • Sequence Length

    • 34

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Listeria, clostridium.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Lipid II

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • There are two dehydroalanines (S5; S33), one dehydrobutyrine (T2), which form one lanthionine (S3-C7) and four β-methyllanthionines (T8-C11; T13-C19; T23-C26; T25-C28).

    • Stereochemistry

    • L

    • Structure

    • Non helix or strand structure

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00036 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00036.

Physicochemical Information

    • Formula

    • C143H246N42O45S7

    • Absent Amino Acids

    • DEFQRWY

    • Common Amino Acids

    • CT

    • Mass

    • 3498.2

    • PI

    • 8.78

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +5

    • Boman Index

    • -12.88

    • Hydrophobicity

    • 0.415

    • Aliphatic Index

    • 71.76

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 250

    • Absorbance 280nm

    • 7.58

    • Polar Residues

    • 18

DRAMP00036

DRAMP00036 chydropathy plot

Comments Information

    • Function

    • Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

    • PTM

    • There are two dehydroalanines (S5; S33), one dehydrobutyrine (T2), which form one lanthionine (S3-C7) and four Beta-methyllanthionines (T8-C11; T13-C19; T23-C26; T25-C28).

    • Medical use

    • Urogenital tract infections and spermicidal activity in-vivo; animal model

Literature Information

  • ·Literature 1

    • Title

    • Structure, expression, and evolution of a gene encoding the precursor of nisin, a small protein antibiotic.

    • Reference

    • J Biol Chem. 1988 Nov 5;263(31):16260-16266.

    • Author

    • Buchman GW, Banerjee S, Hansen JN.
  • ·Literature 2

    • Title

    • The structure of nisin.

    • Reference

    • J Am Chem Soc. 1971 Sep 8;93(18):4634-4635.

    • Author

    • Gross E, Morell JL.
  • ·Literature 3

    • Title

    • Solution structures of nisin A and its two major degradation products determined by n.m.r.

    • Reference

    • Biochem J. 1992 Apr 15;283 (Pt 2):413-420.

    • Author

    • Lian LY, Chan WC, Morley SD, Roberts GC, Bycroft BW, Jackson D.