• DRAMP ID

    • DRAMP00036
    • Peptide Name

    • Nisin A (Bacteriocin; Preclinical)
    • Source

    • Lactococcus lactis subsp. lactis (Streptococcus lactis) (Gram-positive bacteria)
    • Family

    • Belongs to the type A lantibiotic family (Class I bacteriocin)
    • Gene

    • spaN
    • Sequence

    • ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK
    • Sequence Length

    • 34
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Anti-Gram+, Antimicrobial
    • Target Organism

      • Gram-positive bacteria: Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Listeria, clostridium.
    • Hemolytic Activity

      • N/A
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Binding Target

    • Lipid II
    • Linear/Cyclic

    • Cyclic
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Free
    • Other Modifications and Unusual Amino Acids

    • There are two dehydroalanines (S5; S33), one dehydrobutyrine (T2), which form one lanthionine (S3-C7) and four β-methyllanthionines (T8-C11; T13-C19; T23-C26; T25-C28).
    • Stereochemistry

    • L
    • Structure

    • Non helix or strand structure
    • Structure Description

    • Not found
    • DRAMP00036 helical wheel diagram
    • Formula

    • C143H246N42O45S7
    • Absent Amino Acids

    • DEFQRWY
    • Common Amino Acids

    • CT
    • Mass

    • 4087.02
    • PI

    • 8.52
    • Basic Residues

    • 5
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 8
    • Boman Index

    • -12.88
    • Hydrophobicity

    • 0.415
    • Aliphatic Index

    • 71.76
    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 250
    • Absorbance 280nm

    • 7.58
    • Polar Residues

    • 18

DRAMP00036

DRAMP00036 chydropathy plot
    • Function

    • Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
    • PTM

    • There are two dehydroalanines (S5; S33), one dehydrobutyrine (T2), which form one lanthionine (S3-C7) and four Beta-methyllanthionines (T8-C11; T13-C19; T23-C26; T25-C28).
    • Medical use

    • Urogenital tract infections and spermicidal activity in-vivo; animal model
  • ·Literature 1
    • Title

    • Structure, expression, and evolution of a gene encoding the precursor of nisin, a small protein antibiotic.
    • Reference

    • J Biol Chem. 1988 Nov 5;263(31):16260-16266.
    • Author

    • Buchman GW, Banerjee S, Hansen JN.
  • ·Literature 2
    • Title

    • The structure of nisin.
    • Reference

    • J Am Chem Soc. 1971 Sep 8;93(18):4634-4635.
    • Author

    • Gross E, Morell JL.
  • ·Literature 3
    • Title

    • Solution structures of nisin A and its two major degradation products determined by n.m.r.
    • Reference

    • Biochem J. 1992 Apr 15;283 (Pt 2):413-420.
    • Author

    • Lian LY, Chan WC, Morley SD, Roberts GC, Bycroft BW, Jackson D.