• DRAMP ID

    • DRAMP00038
    • Peptide Name

    • Nisin U (Bacteriocin)
    • Source

    • Lactococcus uberis ATCC 27958
    • Family

    • Belongs to the type A lantibiotic family (Class I bacteriocin)
    • Gene

    • nsuA
    • Sequence

    • ITSKSLCTPGCKTGILMTCPLKTATCGCHFG
    • Sequence Length

    • 31
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Anti-Gram+, Antimicrobial
    • Target Organism

      • Gram-positive bacteria: Streptococcus pyogenes, Streptococcus uberis, Streptococcus agalactiae, Streptococcus dysgalactiae, Staphylococcus simulans, Staphylococcus cohnii, Lactococcus lactis, Lactobacillus acidophilus, Streptococcus mitis.
    • Hemolytic Activity

      • N/A
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Binding Target

    • Lipid II
    • Linear/Cyclic

    • Cyclic
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Free
    • Other Modifications and Unusual Amino Acids

    • There are two dihydroalanines (Dha)(S5; T18), one didehydrobutyrine (Dhb)(T2), one lanthionine (S3-C7) and four methyllanthionines (T8-C11; T13-C19; T23-C26; T25-C28).
    • Stereochemistry

    • L
    • Structure

    • Not found
    • Structure Description

    • Not found
    • DRAMP00038 helical wheel diagram
    • Formula

    • C134H226N36O40S6
    • Absent Amino Acids

    • DENQRVWY
    • Common Amino Acids

    • T
    • Mass

    • 3709.15
    • PI

    • 8.51
    • Basic Residues

    • 4
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 7
    • Boman Index

    • -1.63
    • Hydrophobicity

    • 0.448
    • Aliphatic Index

    • 66.13
    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 250
    • Absorbance 280nm

    • 8.33
    • Polar Residues

    • 17

DRAMP00038

DRAMP00038 chydropathy plot
    • Function

    • Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
    • PTM

    • There are two dihydroalanines (Dha)(S5; T18), one didehydrobutyrine (Dhb)(T2), one lanthionine (S3-C7) and four methyllanthionines (T8-C11; T13-C19; T23-C26; T25-C28).
    • Biotechnological use

    • Used as a food preservative.
  • ·Literature 1
    • Title

    • Molecular and genetic characterization of a novel nisin variant produced by Streptococcus uberis.
    • Reference

    • Appl Environ Microbiol. 2006 Feb;72(2):1148-1156.
    • Author

    • Wirawan RE, Klesse NA, Jack RW, Tagg JR.