General Information

    • DRAMP ID

    • DRAMP00038

    • Peptide Name

    • Nisin U (Bacteriocin)

    • Source

    • Lactococcus uberis ATCC 27958

    • Family

    • Belongs to the type A lantibiotic family (Class I bacteriocin)

    • Gene

    • nsuA

    • Sequence

    • ITSKSLCTPGCKTGILMTCPLKTATCGCHFG

    • Sequence Length

    • 31

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: Streptococcus pyogenes, Streptococcus uberis, Streptococcus agalactiae, Streptococcus dysgalactiae, Staphylococcus simulans, Staphylococcus cohnii, Lactococcus lactis, Lactobacillus acidophilus, Streptococcus mitis.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Lipid II

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • There are two dihydroalanines (Dha)(S5; T18), one didehydrobutyrine (Dhb)(T2), one lanthionine (S3-C7) and four methyllanthionines (T8-C11; T13-C19; T23-C26; T25-C28).

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00038 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00038.

Physicochemical Information

    • Formula

    • C134H226N36O40S6

    • Absent Amino Acids

    • DENQRVWY

    • Common Amino Acids

    • T

    • Mass

    • 3173.85

    • PI

    • 8.78

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +4

    • Boman Index

    • -1.63

    • Hydrophobicity

    • 0.448

    • Aliphatic Index

    • 66.13

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 250

    • Absorbance 280nm

    • 8.33

    • Polar Residues

    • 17

DRAMP00038

DRAMP00038 chydropathy plot

Comments Information

    • Function

    • Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

    • PTM

    • There are two dihydroalanines (Dha)(S5; T18), one didehydrobutyrine (Dhb)(T2), one lanthionine (S3-C7) and four methyllanthionines (T8-C11; T13-C19; T23-C26; T25-C28).

    • Biotechnological use

    • Used as a food preservative.

Literature Information

  • ·Literature 1

    • Title

    • Molecular and genetic characterization of a novel nisin variant produced by Streptococcus uberis.

    • Reference

    • Appl Environ Microbiol. 2006 Feb;72(2):1148-1156.

    • Author

    • Wirawan RE, Klesse NA, Jack RW, Tagg JR.