General Information

    • DRAMP ID

    • DRAMP00052

    • Peptide Name

    • Mutacin-2 (Mutacin II mutacin H-29B; Bacteriocin)

    • Source

    • Streptococcus mutans (Gram-positive bacteria)

    • Family

    • Belongs to the type A lantibiotic family (Class I bacteriocin)

    • Gene

    • mutA

    • Sequence

    • NRWWQGVVPTVSYECRMNSWQHVFTCC

    • Sequence Length

    • 27

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacteria: Micrococcus luteus, Staphylococcus aureus, Peptostreptococcus micros, Pediococcus acidilactici, Clostridium sporogenes, Corynebacterium diphtheriae, Actinomyces viscosus, Gardnerella vaginalis, Propionibacterium acnes, Listeria monocytogenes, Mycobacterium smegmatis.
      • Gram-negative bacteria: Campylobacter jejuni, Helicobacter pylori, Neisseria gonorrhoeae.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Rich

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00052 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C147H210N42O39S4

    • Absent Amino Acids

    • ADIKL

    • Common Amino Acids

    • V

    • Mass

    • 3317.78

    • PI

    • 7.97

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +2

    • Boman Index

    • -44.49

    • Hydrophobicity

    • -0.359

    • Aliphatic Index

    • 42.96

    • Half Life

      • Mammalian:1.4 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 18115

    • Absorbance 280nm

    • 696.73

    • Polar Residues

    • 11

DRAMP00052

DRAMP00052 chydropathy plot

Comments Information

    • Function

    • Mutacin-2 inhibits other mutans streptococci as well as many gram-positive exogenous pathogens. Transiently and partially depolarizes the transmembrane electrical potential and pH gradient of susceptible cells, inhibits the uptake of amino acids and depletes the intracellular ATP pool.

    • Biophysicochemical properties

    • pH dependence (Stable from pH 4.0 to 10.0); Temperature dependence (Thermostable).

    • PTM

    • There are one didehydrobutyrine (T25), two lanthionines (S12-C26; S19-C27) and one Beta-methyllanthionine (T10-C15).

Literature Information

  • ·Literature 1

    • Title

    • Biochemical structural analysis of the lantibiotic mutacin II.

    • Reference

    • J Biol Chem. 2000 May 26;275(21):15845-15850.

    • Author

    • Krull RE, Chen P, Novak J, Kirk M, Barnes S, Baker J, Krishna NR, Caufield PW.
  • ·Literature 2

    • Title

    • Mutacin II, a bactericidal antibiotic from Streptococcus mutans.

    • Reference

    • Antimicrob Agents Chemother. 1995 Dec;39(12):2656-2660.

    • Author

    • Chikindas ML, Nov¡k J, Driessen AJ, Konings WN, Schilling KM, Caufield PW.
  • ·Literature 3

    • Title

    • Mutacin H-29B is identical to mutacin II (J-T8).

    • Reference

    • BMC Microbiol. 2006 Apr 20;6:36.

    • Author

    • Nicolas G, Morency H, LaPointe G, Lavoie MC.
  • ·Literature 4

    • Title

    • Isolation and biochemical characterization of a novel lantibiotic mutacin from Streptococcus mutans.

    • Reference

    • J Bacteriol. 1994 Jul;176(14):4316-4320.

    • Author

    • Nov¡k J, Caufield PW, Miller EJ.