General Information

    • DRAMP ID

    • DRAMP00053

    • Peptide Name

    • Nisin F (Bacteriocin; Perclinical)

    • Source

    • Lactococcus lactis F10 (Gram-positive bacteria)

    • Family

    • Belongs to the type A lantibiotic family (Class I bacteriocin)

    • Gene

    • Not found

    • Sequence

    • ITSISLCTPGCKTGALMGCNMKTATCNCSVHVSK

    • Sequence Length

    • 34

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: Staphylococcus aureus, Staphylococcus carnosus, Lactobacillus curvatus N1902, Lactobacillus plantarum, Lactobacillus reuteri.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Lipid II

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • There are two dehydroalanines (S5; S33), one dehydrobutyrine (T2), which form one lanthionine (S3-C7) and four β-methyllanthionines (T8-C11; T13-C19; T23-C26; T25-C28).

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00053 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00053.

Physicochemical Information

    • Formula

    • C140H243N41O46S7

    • Absent Amino Acids

    • DEFQRWY

    • Common Amino Acids

    • CT

    • Mass

    • 3461.14

    • PI

    • 8.78

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +4

    • Boman Index

    • -15.74

    • Hydrophobicity

    • 0.397

    • Aliphatic Index

    • 68.82

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 250

    • Absorbance 280nm

    • 7.58

    • Polar Residues

    • 19

DRAMP00053

DRAMP00053 chydropathy plot

Comments Information

    • Function

    • defense response to Gram-positive bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Characterization of the structural gene encoding nisin F, a new lantibiotic produced by a Lactococcus lactis subsp. lactis isolate from freshwater catfish (Clarias gariepinus).

    • Reference

    • Appl Environ Microbiol. 2008 Jan;74(2):547-549.

    • Author

    • de Kwaadsteniet M, Ten Doeschate K, Dicks LM.