General Information

    • DRAMP ID

    • DRAMP00063

    • Peptide Name

    • Lantibiotic michiganin-A (Bacteriocin)

    • Source

    • Clavibacter michiganensis subsp. Michiganensis (Gram-positive bacteria)

    • Family

    • Belongs to the type B lantibiotic family (Class I bacteriocin)

    • Gene

    • micA

    • Sequence

    • SSSGWLCTLTIECGTIICACR

    • Sequence Length

    • 21

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial

    • Target Organism

    • Clavibacter michiganensis subspecies sepedonicus strain 2136 (MIC=30 pmol/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • All of the threonine residues undergo dehydration, and three of them interact with cysteines via thioether bonds to form methyllanthionine bridges

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00063 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00063.

Physicochemical Information

    • Formula

    • C92H153N25O30S4

    • Absent Amino Acids

    • DFHKMNPQVY

    • Common Amino Acids

    • C

    • Mass

    • 2217.62

    • PI

    • 5.71

    • Basic Residues

    • 1

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 7

    • Net Charge

    • 0

    • Boman Index

    • -3.9

    • Hydrophobicity

    • 0.891

    • Aliphatic Index

    • 97.62

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5750

    • Absorbance 280nm

    • 287.5

    • Polar Residues

    • 12

DRAMP00063

DRAMP00063 chydropathy plot

Comments Information

    • MOA

    • The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

    • PTM

    • Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The 62-67 beta-methyllanthionine thioether bond is oxidized to a sulfoxide. This is followed by membrane translocation and cleavage of the modified precursor

Literature Information

  • ·Literature 1

    • Title

    • Purification, characterization, and gene sequence of michiganin A, an actagardine-like lantibiotic produced by the tomato pathogen Clavibacter michiganensis subsp. michiganensis.

    • Reference

    • Appl Environ Microbiol. 2006 Sep;72(9):5814-5821.

    • Author

    • Holtsmark I, Mantzilas D, Eijsink VG, Brurberg MB.