General Information
-
DRAMP ID
- DRAMP00063
-
Peptide Name
- Lantibiotic michiganin-A (Bacteriocin)
-
Source
- Clavibacter michiganensis subsp. Michiganensis (Gram-positive bacteria)
-
Family
- Belongs to the type B lantibiotic family (Class I bacteriocin)
-
Gene
- micA
-
Sequence
- SSSGWLCTLTIECGTIICACR
-
Sequence Length
- 21
-
Swiss_Prot Entry
- Q09T02
-
Evidence code
- Protein level
Activity Information
-
Biological Activity
- Antibacterial, Antimicrobial
-
Target Organism
- Clavibacter michiganensis subspecies sepedonicus strain 2136 (MIC=30 pmol/ml).
-
Hemolytic Activity
-
- Not found
-
Cytotoxicity
-
- Not included yet
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
-
Other Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Not found
-
Structure Description
- Not found
-
PDB ID
- None
-
-
Physicochemical Information
-
Formula
- C92H153N25O30S4
Absent Amino Acids
- DFHKMNPQVY
Common Amino Acids
- C
Mass
- 2574.55
PI
- 6.02
Basic Residues
- 1
Acidic Residues
- 1
Hydrophobic Residues
- 7
-
Boman Index
- -3.9
Hydrophobicity
- 0.891
Aliphatic Index
- 97.62
Half Life
-
- Mammalian:1.9 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 5750
Absorbance 280nm
- 287.5
Polar Residues
- 12
DRAMP00063

Comments Information
MOA
- The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
PTM
- Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The 62-67 beta-methyllanthionine thioether bond is oxidized to a sulfoxide. This is followed by membrane translocation and cleavage of the modified precursor
Literature Information
- ·Literature 1
-
Title
- Purification, characterization, and gene sequence of michiganin A, an actagardine-like lantibiotic produced by the tomato pathogen Clavibacter michiganensis subsp. michiganensis.
-
Pubmed ID
- 16957199
-
Reference
- Appl Environ Microbiol. 2006 Sep;72(9):5814-5821.
-
Author
- Holtsmark I, Mantzilas D, Eijsink VG, Brurberg MB.