• DRAMP ID

    • DRAMP00063
    • Peptide Name

    • Lantibiotic michiganin-A (Bacteriocin)
    • Source

    • Clavibacter michiganensis subsp. Michiganensis (Gram-positive bacteria)
    • Family

    • Belongs to the type B lantibiotic family (Class I bacteriocin)
    • Gene

    • micA
    • Sequence

    • SSSGWLCTLTIECGTIICACR
    • Sequence Length

    • 21
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial
    • Target Organism

      • Clavibacter michiganensis subspecies sepedonicus strain 2136 (MIC=30 pmol/ml).
    • Hemolytic activity

    • Unknown
    • Binding Traget

    • Unknow
    • Structure

    • Unknow
    • Structure Description

    • Unknow
    • PDB ID

    • Unknow
    • DRAMP00063 helical wheel diagram
    • Formula

    • C92H153N25O30S4
    • Absent Amino Acids

    • DFHKMNPQVY
    • Common Amino Acids

    • C
    • Mass

    • 2574.55
    • PI

    • 6.02
    • Basic Residues

    • 1
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 7
    • Boman Index

    • -3.9
    • Hydrophobicity

    • 89.05
    • Aliphatic Index

    • 97.62
    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 5750
    • Absorbance 280nm

    • 287.5
    • Polar Residues

    • 12

DRAMP00063

DRAMP00063 chydropathy plot
    • MOA

    • The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
    • PTM

    • Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The 62-67 beta-methyllanthionine thioether bond is oxidized to a sulfoxide. This is followed by membrane translocation and cleavage of the modified precursor
  • Literature 1
    • Reference

    • Appl Environ Microbiol. 2006 Sep;72(9):5814-5821.
    • Author

    • Holtsmark I, Mantzilas D, Eijsink VG, Brurberg MB.
    • Title

    • Purification, characterization, and gene sequence of michiganin A, an actagardine-like lantibiotic produced by the tomato pathogen Clavibacter michiganensis subsp. michiganensis.