General Information
-
DRAMP ID
- DRAMP00074
-
Peptide Name
- Enterocin P (Pediocin-like peptide; Bacteriocin)
-
Source
- Enterococcus faecium P13 (Gram-positive bacteria)
-
Family
- Belongs to the class IIa bacteriocin
-
Gene
- entP
-
Sequence
- ATRSYGNGVYCNNSKCWVNWGEAKENIAGIVISGWASGLAGMGH
-
Sequence Length
- 44
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+
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Target Organism
-
- [Ref.9361419]Gram-positive bacteria: Pediococcus acidilactici (MIC=69 ng/ml), Pediococcus pentosaceus FBB61 (MIC=136 ng/ml), P. pntosaceus FBB63 (MIC=18 ng/ml), Lactococcus lactis BB24 (MIC=22 ng/ml), Enterococcus faecium (MIC=2 ng/ml), E. faecalis (MIC=238 ng/ml), Staphylococcus carnosus (MIC=139 ng/ml), Bacillus cereus (MIC=286 ng/ml), Clostridium sporogenes (MIC=4 ng/ml), Clostridium tyrobutyricum 3, 5CT (MIC=559 ng/ml), C. tyrobutyricum 1754 (MIC=412 ng/ml), Propionibacteruim sp P4 (MIC=37 ng/ml), Propionibacteruim sp P6 (MIC=26 ng/ml), Clostridium tyrobutyricum (MIC=4 ng/ml), Clostridium botulinum (MIC=259 ng/ml), Listeria monocytogenes 7973 (MIC=35 ng/ml), L. monocytogenes LI5sv1/2 (MIC=33 ng/ml), L. monocytogenes 5105 (MIC=18 ng/ml), L. monocytogenes L11sv4 (MIC=39 ng/ml), L. monocytogenes Scott A (MIC=125 ng/ml), Listeria innocua (MIC=395 ng/ml), Streptococcus aureus 137 (MIC=190 ng/ml), S. aureus 196E (MIC=407 ng/ml), S. aureus 349 (MIC=221 ng/ml), S. aureus 361 (MIC=294 ng/ml), S. aureus 472 (MIC=269 ng/ml), Lactobacillus curvatus (MIC=17 ng/ml), L. fermentum (MIC=1 ng/ml), L. sake (MIC=144 ng/ml).
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Bridge
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP00074.
Physicochemical Information
-
Formula
- C202H303N59O61S3
Absent Amino Acids
- DFPQ
Common Amino Acids
- G
Mass
- 4630.17
PI
- 8.07
Basic Residues
- 4
Acidic Residues
- 2
Hydrophobic Residues
- 15
Net Charge
- +2
-
Boman Index
- -33.68
Hydrophobicity
- -0.15
Aliphatic Index
- 66.59
Half Life
-
- Mammalian:4.4 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 19605
Absorbance 280nm
- 455.93
Polar Residues
- 22
DRAMP00074
Comments Information
Function
- Bacteriocin that inhibits the growth of wide range of Gram-positive bacterial species. Does not inhibit the growth of several strains of Gram-negative bacteria. No hemolytic activity against calf blood.
Biophysicochemical properties
- pH dependence (Stable between pH 3.0 and pH 7.0); Temperature dependence (Thermostable, retains activity after heating at 100 and 121 degrees Celsius).
PTM
- Contains one disulfide bond 11-16.
Literature Information
- ·Literature 1
-
Title
- Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum.
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Pubmed ID
- 9361419
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Reference
- Appl Environ Microbiol. 1997 Nov;63(11):4321-4330.
-
Author
- Cintas LM, Casaus P, H¥varstein LS, Hern¡ndez PE, Nes IF.
- ·Literature 2
-
Title
- Single nucleotide polymorphism analysis of the enterocin P structural gene of Enterococcus faecium strains isolated from nonfermented animal foods.
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Pubmed ID
- 17103378
-
Reference
- Mol Nutr Food Res. 2006 Dec;50(12):1229-1238.
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Author
- Arlindo S, Calo P, Franco C, Prado M, Cepeda A, Barros-Vel¡zquez J.