General Information
-
DRAMP ID
- DRAMP00090
-
Peptide Name
- Carnobacteriocin B2 (CbnB2; Bacteriocin)
-
Source
- Carnobacterium piscicola LV18B (Gram-positive bacteria)
-
Family
- Belongs to the class IIa bacteriocin
-
Gene
- cbnB2
-
Sequence
- VNYGNGVSCSKTKCSVNWGQAFQERYTAGINSFVSGVASGAGSIGRRP
-
Sequence Length
- 48
-
UniProt Entry
- P38580
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+
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Target Organism
-
- [Ref.8163526]Gram-positive bacteria: Lactobacillus plantarum ATCC 4008 (MIC=2 AU/μg), Pediococcus parvulus ATCC 19371 (MIC=8 AU/μg), Listeria monocytogenes ATCC 15313 (MIC=32 AU/μg), Listeria innocua ATCC 33090 (MIC=16 AU/μg), Enterococcus faecium ATCC 11576 (MIC=16 AU/μg), Enterococcus faecalis ATCC 19433 (MIC=16 AU/μg), Enterococcus faecium ATCC 19434 (MIC=4 AU/μg).
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- [Ref.8163526 & Ref.10569926] CbnB2 can easily form a disulfide bond between Cys9 and Cys14
-
Stereochemistry
- L
-
Structure
- Alpha helix (1 helices; 24 residues)
-
Structure Description
- CbnB2 forms a disulfide bond and that this peptide has full antimicrobial activity. NMR results indicate that CbnB2 in TFE has a well-defined central helical structure (residues 18-39) but a disordered N terminus. And the central helical structure is conserved.
-
Helical Wheel Diagram
-
PDB ID
- 1CW5 resolved by NMR.
-
Predicted Structure
- There is no predicted structure for DRAMP00090.
Physicochemical Information
-
Formula
- C213H332N66O68S2
Absent Amino Acids
- DHLM
Common Amino Acids
- G
Mass
- 4969.5
PI
- 9.7
Basic Residues
- 5
Acidic Residues
- 1
Hydrophobic Residues
- 14
Net Charge
- +4
-
Boman Index
- -73.88
Hydrophobicity
- -0.277
Aliphatic Index
- 54.79
Half Life
-
- Mammalian:100 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 8605
Absorbance 280nm
- 183.09
Polar Residues
- 25
DRAMP00090
Comments Information
Function
- Has antibacterial activity against Listeria and Enterococcus.
PTM
- Contains one disulfide bond 9-14.
Literature Information
- ·Literature 1
-
Title
- Chemical and genetic characterization of bacteriocins produced by Carnobacterium piscicola LV18B.
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Pubmed ID
- 8163526
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Reference
- J Biol Chem 1994; 269: 12204-12211.
-
Author
- Quadri LE et al Stiles ME.
- ·Literature 2
-
Title
- Characteristics and genetic determinants of bacteriocin activities produced by Carnobacterium piscicola CP5 isolated from cheese.
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Pubmed ID
- 9353214
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Reference
- Curr Microbiol. 1997 Dec;35(6):319-326.
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Author
- Herbin S, Mathieu F, Brul© F, Branlant C, Lefebvre G, Lebrihi A.
- ·Literature 3
-
Title
- Solution structure of carnobacteriocin B2 and implications for structure-activity relationships among type IIa bacteriocins from lactic acid bacteria.
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Pubmed ID
- 10569926
-
Reference
- Biochemistry. 1999 Nov 23;38(47):15438-15447.
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Author
- Wang Y, Henz ME, Gallagher NL, Chai S, Gibbs AC, Yan LZ, Stiles ME, Wishart DS, Vederas JC.