General Information

    • DRAMP ID

    • DRAMP00101

    • Peptide Name

    • Sakacin P (Sakacin 674; Pediocin-like peptide; Bacteriocin)

    • Source

    • Lactobacillus sakei Lb674 (Gram-positive bacteria)

    • Family

    • Belongs to the class IIa bacteriocin

    • Gene

    • sakP

    • Sequence

    • KYYGNGVHCGKHSCTVDWGTAIGNIGNNAAANWATGGNAGWNK

    • Sequence Length

    • 43

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: Listeria monocytogenes and ivanovvi, Enterococcus faecalis, Carnobacterium sp and Brocothrix thermosphacta.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Combine helix and strand structure

    • Structure Description

    • Structures in TFE and DPC were determined (Uteng M et al 2003 Biochemistry 42: 11417-26). The N-terminal region formed a 3-stranded beta sheet-like structure, while a well defined helical region was found between residues 18-33. The beta-like structure and the helical region are not packed together.

    • Helical Wheel Diagram

    • DRAMP00101 helical wheel diagram
  • 1OG7-> 

    • Predicted Structure

Physicochemical Information

    • Formula

    • C192H280N60O59S2

    • Absent Amino Acids

    • EFLMPQR

    • Common Amino Acids

    • G

    • Mass

    • 4436.82

    • PI

    • 8.79

    • Basic Residues

    • 5

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +4

    • Boman Index

    • -45.77

    • Hydrophobicity

    • -0.574

    • Aliphatic Index

    • 45.58

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 19605

    • Absorbance 280nm

    • 466.79

    • Polar Residues

    • 24

DRAMP00101

DRAMP00101 chydropathy plot

Comments Information

    • Function

    • Has bactericidal activity.

    • PTM

    • Contains one disulfide bond 9-14.

Literature Information

  • ·Literature 1

    • Title

    • Purification and cloning of sakacin 674, a bacteriocin from Lactobacillus sakei Lb674.

    • Reference

    • FEMS Microbiol Lett. 1994 Jan 15;115(2-3):143-149.

    • Author

    • Holck AL, Axelsson L, H¼hne K, Kröckel L.
  • ·Literature 2

    • Title

    • Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P and a sakacin P variant that is structurally stabilized by an inserted C-terminal disulfide bridge.

    • Reference

    • Biochemistry. 2003 Oct 7;42(39):11417-11426.

    • Author

    • Uteng M, Hauge HH, Markwick PR, Fimland G, Mantzilas D, Nissen-Meyer J, Muhle-Goll C.