General Information
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+
-
Target Organism
-
- Gram-positive bacteria: Pediococcus pentosaceus Pac 1.0 (MIC=5 nM), Lactobacillus plantarum 965 (MIC=7 nM), Lactobacillus casei subsp.casei NCDO 161 (MIC=20 nM), Lactobacillus casei NCDO 2713 (MIC=150 nM), Lactobacillus sakei NCDO 2714 (MIC=4 nM), Lactobacillus viridescens NCDO 1655 (MIC=2 nM), Pediococcus pentosaceus NCDO 990 (MIC=30 nM), Lactobacillus sakei 706 (MIC=100 nM), Pediococcus acidilactici CH (MIC=10 nM), Lactobacillus curvatus 89 (MIC=5 nM). [PlnE:PlnF=1:1]
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- There is a possible hydrogen bond/salt bridge between Asp17 and Arg26
-
Stereochemistry
- L
-
Structure
- Alpha helix (3 helices; 19 residues)
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Structure Description
- In DPC micelles, PlnE has an N-terminal alpha-helix (residues 10-21), and a C-terminal alpha-helix-like structure (residues 25-31). Both helices in plnE are amphiphilic, while the helix in plnF is polar in its N-terminal half and amphiphilic in its C-terminal half. PlnE has two GxxxG motifs which are putative helix-helix interaction motifs, one at residues 5 to 9 and one at residues 20 to 24.
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Helical Wheel Diagram
-
PDB ID
- 2JUI resolved by NMR.
-
Predicted Structure
- There is no predicted structure for DRAMP00126.
Physicochemical Information
-
Formula
- C158H259N51O42
Absent Amino Acids
- CEMPQTW
Common Amino Acids
- G
Mass
- 3545.11
PI
- 11.57
Basic Residues
- 7
Acidic Residues
- 1
Hydrophobic Residues
- 13
Net Charge
- +6
-
Boman Index
- -51.8
Hydrophobicity
- 0.067
Aliphatic Index
- 100.3
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 1490
Absorbance 280nm
- 46.56
Polar Residues
- 12
DRAMP00126
Comments Information
Comment
- No comments found on DRAMP database
Literature Information
- ·Literature 1
-
Title
- The gene encoding plantaricin A, a bacteriocin from Lactobacillus plantarum C11, is located on the same transcription unit as an agr-like regulatory system.
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Pubmed ID
- 8117074
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Reference
- Appl Environ Microbiol. 1994 Jan;60(1):160-166.
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Author
- Diep DB, Håvarstein LS, Nissen-Meyer J, Nes IF.
- ·Literature 2
-
Title
- Three-dimensional structure of the two peptides that constitute the two-peptide bacteriocin plantaricin EF.
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Pubmed ID
- 18555030
-
Reference
- Biochim Biophys Acta. 2008 Nov;1784(11):1711-1719.
-
Author
- Fimland N, Rogne P, Fimland G, Nissen-Meyer J, Kristiansen P.E.