General Information
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+
-
Target Organism
-
- Gram-positive bacteria: Pediococcus pentosaceus Pac 1.0 (MIC=50 nM), Lactobacillus plantarum 965 (MIC=0.1 nM), L. sake NCDO 2714 (MIC=50 nM), Leptostylus viridescens NCDO 1655 (MIC=5 nM). [PlnJ:PlnK=1:1]
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Alpha helix
-
Structure Description
- In DPC micelles, PlnJ has an N-terminal amphiphilic alpha-helix between Trp-3 and Tyr-15. Crucial residues: Gly-13 in PlnJ is very sensitive, giving more than a 100-fold reduction in activity when large residues replace glycine. Gly-20 in PlnJ was replaced, the activity was reduced less than 10-fold.
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Helical Wheel Diagram
-
Predicted Structure
- There is no predicted structure for DRAMP00128.
Physicochemical Information
-
Formula
- C133H198N42O34
Absent Amino Acids
- CHMPQTV
Common Amino Acids
- GR
Mass
- 2929.3
PI
- 10.93
Basic Residues
- 6
Acidic Residues
- 2
Hydrophobic Residues
- 9
Net Charge
- +4
-
Boman Index
- -70.24
Hydrophobicity
- -0.932
Aliphatic Index
- 43.2
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 12490
Absorbance 280nm
- 520.42
Polar Residues
- 8
DRAMP00128
Comments Information
Function
- A 1
Literature Information
- ·Literature 1
-
Title
- Antagonistic activity of Lactobacillus plantarum C11: two new two-peptide bacteriocins, plantaricins EF and JK, and the induction factor plantaricin A.
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Pubmed ID
- 9603847
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Reference
- Appl Environ Microbiol. 1998 Jun;64(6):2269-2272.
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Author
- Anderssen EL, Diep DB, Nes IF, Eijsink VG, Nissen-Meyer J.
- ·Literature 2
-
Title
- Three-dimensional structure of the two-peptide bacteriocin plantaricin JK.
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Pubmed ID
- 19538999
-
Reference
- Peptides. 2009 Sep;30(9):1613-1621.
-
Author
- Rogne P, Haugen C, Fimland G, Nissen-Meyer J, Kristiansen PE.