General Information

    • DRAMP ID

    • DRAMP00129

    • Peptide Name

    • Plantaricin K (PlnK; Bacteriocin)

    • Source

    • Lactobacillus plantarum C11 (Gram-positive bacteria)

    • Family

    • Belongs to the class IIb bacteriocin

    • Gene

    • plnK

    • Sequence

    • RRSRKNGIGYAIGYAFGAVERAVLGGSRDYNK

    • Sequence Length

    • 32

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: Pediococcus pentosaceus Pac 1.0 (MIC=50 nM), Lactobacillus plantarum 965 (MIC=0.1 nM), L. sake NCDO 2714 (MIC=50 nM), Leptostylus viridescens NCDO 1655 (MIC=5 nM). [PlnJ:PlnK=1:1]

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • In DPC micelles, PlnK forms a central amphiphilic alpha-helix between Gly-9 and Leu-24. Crucial residues: Gly-17 in PlnK is very sensitive, giving more than a 100-fold reduction in activity when large residues replace glycine. Gly-7, Gly-9, Gly-24 and Gly-25 in PlnK were replaced, the activity was reduced less than 10-fold.

    • Helical Wheel Diagram

    • DRAMP00129 helical wheel diagram
  • 2KEG-> 

    • Predicted Structure

    • There is no predicted structure for DRAMP00129.

Physicochemical Information

    • Formula

    • C153H245N51O44

    • Absent Amino Acids

    • CHMPQTW

    • Common Amino Acids

    • G

    • Mass

    • 3502.94

    • PI

    • 10.52

    • Basic Residues

    • 7

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +5

    • Boman Index

    • -83.03

    • Hydrophobicity

    • -0.656

    • Aliphatic Index

    • 67.19

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 4470

    • Absorbance 280nm

    • 144.19

    • Polar Residues

    • 13

DRAMP00129

DRAMP00129 chydropathy plot

Comments Information

    • Function

    • A 1

Literature Information

  • ·Literature 1

    • Title

    • Antagonistic activity of Lactobacillus plantarum C11: two new two-peptide bacteriocins, plantaricins EF and JK, and the induction factor plantaricin A.

    • Reference

    • Appl Environ Microbiol. 1998 Jun;64(6):2269-2272.

    • Author

    • Anderssen EL, Diep DB, Nes IF, Eijsink VG, Nissen-Meyer J.
  • ·Literature 2

    • Title

    • Three-dimensional structure of the two-peptide bacteriocin plantaricin JK.

    • Reference

    • Peptides. 2009 Sep;30(9):1613-1621.

    • Author

    • Rogne P, Haugen C, Fimland G, Nissen-Meyer J, Kristiansen PE.