• DRAMP ID

    • DRAMP00129
    • Peptide Name

    • Plantaricin K (PlnK; Bacteriocin)
    • Source

    • Lactobacillus plantarum C11 (Gram-positive bacteria)
    • Family

    • Belongs to the class IIb bacteriocin
    • Gene

    • plnK
    • Sequence

    • RRSRKNGIGYAIGYAFGAVERAVLGGSRDYNK
    • Sequence Length

    • 32
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial
    • Target Organism

      • Gram-positive bacteria: Pediococcus pentosaceus Pac 1.0 (MIC=50 nM), Lactobacillus plantarum 965 (MIC=0.1 nM), L. sake NCDO 2714 (MIC=50 nM), Leptostylus viridescens NCDO 1655 (MIC=5 nM). [PlnJ:PlnK=1:1]
    • Hemolytic activity

    • Unknown
    • Binding Traget

    • Unknow
    • Structure

    • Alpha helix
    • Structure Description

    • In DPC micelles, PlnK forms a central amphiphilic alpha-helix between Gly-9 and Leu-24. Crucial residues: Gly-17 in PlnK is very sensitive, giving more than a 100-fold reduction in activity when large residues replace glycine. Gly-7, Gly-9, Gly-24 and Gly-25 in PlnK were replaced, the activity was reduced less than 10-fold.
    • DRAMP00129 helical wheel diagram
    • 2KEG-> 
    • Formula

    • C153H245N51O44
    • Absent Amino Acids

    • CHMPQTW
    • Common Amino Acids

    • G
    • Mass

    • 4056.06
    • PI

    • 10.98
    • Basic Residues

    • 7
    • Acidic Residues

    • 2
    • Hydrophobic Residues

    • 10
    • Boman Index

    • -83.03
    • Hydrophobicity

    • -65.63
    • Aliphatic Index

    • 67.19
    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 4470
    • Absorbance 280nm

    • 144.19
    • Polar Residues

    • 13

Amino Acid Distribution

DRAMP00129 chydropathy plot
    • A 1

    • 1 ratio of PlnK and PlanJ is at least 1000 times more active than the individual peptide.
  • Literature 1
    • Reference

    • Appl Environ Microbiol. 1998 Jun;64(6):2269-2272.
    • Author

    • Anderssen EL, Diep DB, Nes IF, Eijsink VG, Nissen-Meyer J.
    • Title

    • Antagonistic activity of Lactobacillus plantarum C11: two new two-peptide bacteriocins, plantaricins EF and JK, and the induction factor plantaricin A.
  • Literature 2
    • Reference

    • Peptides. 2009 Sep;30(9):1613-1621.
    • Author

    • Rogne P, Haugen C, Fimland G, Nissen-Meyer J, Kristiansen PE.
    • Title

    • Three-dimensional structure of the two-peptide bacteriocin plantaricin JK.