General Information

    • DRAMP ID

    • DRAMP00194

    • Peptide Name

    • Capistruin (Bacteriocin)

    • Source

    • Burkholderia thailandensis E264 (Gram-negative bacteria)

    • Family

    • Belongs to the class I microcin

    • Gene

    • Not found

    • Sequence

    • GTPGFQTPDARVISRFGFN

    • Sequence Length

    • 19

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial

    • Target Organism

    • Burkholderia and Pseudomonas strains

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Beta strand

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00194 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00194.

Physicochemical Information

    • Formula

    • C93H139N27O27

    • Absent Amino Acids

    • CEHKLMWY

    • Common Amino Acids

    • FG

    • Mass

    • 2067.29

    • PI

    • 9.6

    • Basic Residues

    • 2

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +1

    • Boman Index

    • -36.75

    • Hydrophobicity

    • -0.379

    • Aliphatic Index

    • 41.05

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 7

DRAMP00194

DRAMP00194 chydropathy plot

Comments Information

    • Discovered by genome mining based on the known MccJ25-gene coding cluster McjABCD. Capistruin is endoded by the capABCD gene cluster, where capA encodes the precursor polypeptide, capB cleaves the presursor and is likely to promote the cyclization, capC encodes an enzyme that activates the side chain of Asp9 for cyclization, and capD encodes a immunity protein. Showed activity against closely related Burkholderia and Pseudomonas strains. Different from the lasso structure of MccJ25, where the N-terminal Gly forms an amide bond with the carboxyl group of Glu8, capistruin forms a 9-residue macrolactam ring formed between NH of Gly1 and the carboxylic side chain of Asp9. In addition, Arg15 is reponsible for the trapping of the tail in the ring structure.

Literature Information

  • ·Literature 1

    • Title

    • Isolation and structural characterization of capistruin, a lasso peptide predicted from the genome sequence of Burkholderia thailandensis E264.

    • Reference

    • J Am Chem Soc. 2008 Aug 27;130(34):11446-11454.

    • Author

    • Knappe TA, Linne U, Zirah S, Rebuffat S, Xie X, Marahiel MA.