General Information

    • DRAMP ID

    • DRAMP00232

    • Peptide Name

    • Cypemycin (Bacteriocin)

    • Source

    • Streptomyces sp. OH-4156 (Gram-positive bacteria)

    • Family

    • Belongs to the linaridin family

    • Gene

    • cypA

    • Sequence

    • ATPATPTVAQFVIQGSTICLVC

    • Sequence Length

    • 22

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial

    • Target Organism

    • Micrococcus luteus (MIC=0.2 µg/ml)

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • [Ref.7802859] IC50 = 1.3 μg/mL against P388 leukemia cells in vitro.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • [Ref. 10.1016/S0040-4039(00)78407-3] Dimethylation on Ala1

    • C-terminal Modification

    • [Ref. 10.1016/S0040-4039(00)78407-3] Decarboxylation and Cyclization on D-Cys64

    • Nonterminal Modifications and Unusual Amino Acids

    • [Ref. 10.1016/S0040-4039(00)78407-3] ①Thr2, Thr5, Thr7 and Thr17 converts to (E)-2,3-didehydrobutyrine. ②Ile13 and Ile18 converts to L-allo-isoleucine. ③Maturation involves the enzymatic conversion of Cys-19 into 2,3-didehydroalanine followed by formation of a thioether bond with Cys-22. The C-terminal meso-lanthionine then undergoes decarboxylation.

    • Stereochemistry

    • [Ref. 10.1016/S0040-4039(00)78407-3] Mixed (D-Cys19)

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00232 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00232.

Physicochemical Information

    • Formula

    • C98H162N24O30S2

    • Absent Amino Acids

    • DEHKMNRWY

    • Common Amino Acids

    • T

    • Mass

    • 2220.63

    • PI

    • 5.55

    • Basic Residues

    • 0

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • 0

    • Boman Index

    • 14.03

    • Hydrophobicity

    • 1.109

    • Aliphatic Index

    • 106.36

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.95

    • Polar Residues

    • 8

DRAMP00232

DRAMP00232 chydropathy plot

Comments Information

    • Function

    • Antibiotic peptide structurally related to lantibiotics. Has cytotoxic activity against mouse P388 leukemia cells (IC50=1.3 μg/ml).

Literature Information

  • ·Literature 1

    • Title

    • A new antibiotic, cypemycin. Taxonomy, fermentation, isolation and biological characteristics.

    • Reference

    • J Antibiot (Tokyo). 1993 Nov;46(11):1666-1671.

    • Author

    • Komiyama K, Otoguro K, Segawa T, Shiomi K, Yang H, Takahashi Y, Hayashi M, Otani T, Omura S.
  • ·Literature 2

    • Title

    • STRUCTURE OF CYPEMYCIN, A NEW PEPTIDE ANTIBIOTIC.

    • Reference

    • Tetrahedron Lett. 1994 Oct;35(43):8001-8004.

    • Author

    • Minami Y, Yoshida K, Azuma R, Urakawa A, Kawauchi T, Otani T, Komiyama K, Omura S