General Information

    • DRAMP ID

    • DRAMP00234

    • Peptide Name

    • Trifolitoxin (TFX; Bacteriocin)

    • Source

    • Rhizobium leguminosarum bv. Trifolii T24 (Gram-negative bacteria)

    • Family

    • Not found

    • Gene

    • tfxA

    • Sequence

    • DIGGSRQGCVA

    • Sequence Length

    • 11

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00234 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00234.

Physicochemical Information

    • Formula

    • C41H71N15O16S

    • Absent Amino Acids

    • EFHKLMNPTWY

    • Common Amino Acids

    • G

    • Mass

    • 1062.17

    • PI

    • 5.83

    • Basic Residues

    • 1

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 3

    • Net Charge

    • 0

    • Boman Index

    • -17.71

    • Hydrophobicity

    • -0.046

    • Aliphatic Index

    • 70.91

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 5

DRAMP00234

Comments Information

    • Function

    • Antibiotic whose production provides the bacteria a mechanism of host root nodule competitiveness with other invading inefficient strains.

    • PTM

    • Synthesized ribosomally as a prepeptide that is post-translationally modified to the active peptide. TfxB, TfxD and TfxF are all required for this processing.The chromophore is probably a six-membered ring derived from the cyclization of Gln-38.

    • Maturation of thiazole and oxazole containing antibiotics involves the enzymic condensation of a Cys, Ser or Thr with the alpha-carbonyl of the preceding amino acid to form a thioether or ether bond, then dehydration to form a double bond with the alpha-amino nitrogen. Thiazoline or oxazoline rings are dehydrogenated to form thiazole or oxazole rings.

    • Biophysicochemical properties

    • pH dependence (Sensitive to extremes of pH); Temperature dependence (Thermostable).

Literature Information

  • ·Literature 1

    • Title

    • DNA sequence and mutational analysis of genes involved in the production and resistance of the antibiotic peptide trifolitoxin.

    • Reference

    • J Bacteriol. 1993 Jun;175(12):3693-3702.

    • Author

    • Breil BT, Ludden PW, Triplett EW.