General Information

    • DRAMP ID

    • DRAMP00290

    • Peptide Name

    • Defensin Lc-def (Plant defensin)

    • Source

    • Lens culinaris subsp. culinaris (Cultivated lentil) (Lens esculenta)

    • Family

    • Belongs to the DEFL family

    • Gene

    • Not found

    • Sequence

    • KTCENLSDSFKGPCIPDGNCNKHCKEKEHLLSGRCRDDFRCWCTRNC

    • Sequence Length

    • 47

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antifungal

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Combine helix and strand structure

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00290 helical wheel diagram

    • PDB ID

    • 2LJ7 resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP00290.

Physicochemical Information

    • Formula

    • C223H353N73O71S8

    • Absent Amino Acids

    • AMQVY

    • Common Amino Acids

    • C

    • Mass

    • 5449.18

    • PI

    • 8.2

    • Basic Residues

    • 11

    • Acidic Residues

    • 7

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +4

    • Boman Index

    • -152.93

    • Hydrophobicity

    • -1.064

    • Aliphatic Index

    • 33.19

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 6000

    • Absorbance 280nm

    • 130.43

    • Polar Residues

    • 20

DRAMP00290

DRAMP00290 chydropathy plot

Comments Information

    • Function

    • Has antifungal activity against the phytopathogenic fungus Aspergillus niger VKM F-2259, but not against A.alternatia VKM F- 3047. Does not inhibit trypsin or chymotrypsin.

    • PTM

    • Contains four disulfide bonds (By similarity).

Literature Information

  • ·Literature 1

    • Title

    • A novel defensin from the lentil Lens culinaris seeds.

    • Reference

    • Biochem Biophys Res Commun. 2008 Jul 11;371(4):860-865.

    • Author

    • Finkina EI, Shramova EI, Tagaev AA, Ovchinnikova TV.
  • ·Literature 2

    • Title

    • Solution structure of defensin Lc-def from germinated lentil (Lens Culinaris) seeds.

    • Reference

    • To be Published.

    • Author

    • Shenkarev Z, Mineev K, Gizatullina A, Finkina E, Arseniev A, Ovchinnikova T.