• DRAMP ID

    • DRAMP00302
    • Peptide Name

    • Malanin chain B (Plant defensin)
    • Source

    • Malania oleifera
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • DETXTDEEFN
    • Sequence Length

    • 10
    • Protein Existence

    • Protein level
    • Biological Activity

    • Anti-cancer, Cytotoxic
    • Target Organism

      • Cancer cell lines: HeLa (IC50=0.15±0.08 nM), PC-12 (IC50=7.71±0.24 nM), MCF-7 (IC50=11.20±0.02 nM), K562 (IC50=15.80±0.09 nM).
      • Non-cancer cell lines: Vero (IC50=2.79±0.05 nM) and Madin-Darby canine kidney cells (IC50=3.92±0.01 nM).
    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Not included yet
    • N-terminal Modification

    • Not included yet
    • C-terminal Modification

    • Not included yet
    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet
    • Stereochemistry

    • Not included yet
    • Structure

    • Not found
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP00302 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP00302.
    • Formula

    • C44H60N10O22
    • Absent Amino Acids

    • ACGHIKLMPQRSVWY
    • Common Amino Acids

    • E
    • Mass

    • 1210.36
    • PI

    • 3.39
    • Basic Residues

    • 0
    • Acidic Residues

    • 5
    • Hydrophobic Residues

    • 1
    • Net Charge

    • -5
    • Boman Index

    • -46.67
    • Hydrophobicity

    • -1.96
    • Aliphatic Index

    • 0
    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 0
    • Absorbance 280nm

    • 0
    • Polar Residues

    • 3

DRAMP00302

    • Function

    • Significantly inhibits growth and induces an apoptotic response in HeLa cells through cell-cycle arrest at S-phase. Exhibits highly cytotoxic activities against cancer cell and non-cancer cell lines.
    • Subunit structure

    • Heterodimer of an A chain and a B chain, which are crosslinked by one or more disulfide bonds.
    • Toxic dose

    • LD50 values are 26.22 µg/kg by intraperitoneal injection and 43.11 mg/kg by intragingival injection into IRC strain mice.
  • ·Literature 1
    • Title

    • Purification, characterization and cytotoxicity of malanin, a novel plant toxin from the seeds of Malania oleifera.
    • Reference

    • Toxicon. 2009 Aug;54(2):121-127.
    • Author

    • Yuan Y, Dai X, Wang D, Zeng X.