General Information

    • DRAMP ID

    • DRAMP00302

    • Peptide Name

    • Malanin chain B (Plant defensin)

    • Source

    • Malania oleifera

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • DETXTDEEFN

    • Sequence Length

    • 10

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Anti-cancer, Cytotoxic

    • Target Organism

      • Cancer cell lines: HeLa (IC50=0.15±0.08 nM), PC-12 (IC50=7.71±0.24 nM), MCF-7 (IC50=11.20±0.02 nM), K562 (IC50=15.80±0.09 nM).
      • Non-cancer cell lines: Vero (IC50=2.79±0.05 nM) and Madin-Darby canine kidney cells (IC50=3.92±0.01 nM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00302 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00302.

Physicochemical Information

    • Formula

    • C44H60N10O22

    • Absent Amino Acids

    • ACGHIKLMPQRSVWY

    • Common Amino Acids

    • E

    • Mass

    • 1210.36

    • PI

    • 3.39

    • Basic Residues

    • 0

    • Acidic Residues

    • 5

    • Hydrophobic Residues

    • 1

    • Net Charge

    • -5

    • Boman Index

    • -46.67

    • Hydrophobicity

    • -1.96

    • Aliphatic Index

    • 0

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP00302

Comments Information

    • Function

    • Significantly inhibits growth and induces an apoptotic response in HeLa cells through cell-cycle arrest at S-phase. Exhibits highly cytotoxic activities against cancer cell and non-cancer cell lines.

    • Subunit structure

    • Heterodimer of an A chain and a B chain, which are crosslinked by one or more disulfide bonds.

    • Toxic dose

    • LD50 values are 26.22 µg/kg by intraperitoneal injection and 43.11 mg/kg by intragingival injection into IRC strain mice.

Literature Information

  • ·Literature 1

    • Title

    • Purification, characterization and cytotoxicity of malanin, a novel plant toxin from the seeds of Malania oleifera.

    • Reference

    • Toxicon. 2009 Aug;54(2):121-127.

    • Author

    • Yuan Y, Dai X, Wang D, Zeng X.