General Information
-
DRAMP ID
- DRAMP00333
-
Peptide Name
- Antiviral protein DAP-32 (Ribosome-inactivating protein; Plant defensin)
-
Source
- Dianthus caryophyllus (Carnation) (Clove pink)
-
Family
- Belongs to the ribosome-inactivating protein family (Type 1 RIP subfamily)
-
Gene
- Not found
-
Sequence
- AVKTITLNLVSPSANRYATFLTEIRDNVRXRSLDYSHSGIDVIGAPSSRDSXLNINFQSP
-
Sequence Length
- 60
-
UniProt Entry
- P24477
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antiviral
-
Target Organism
- Syncytkia (IC50=0.76 nM), viral coreprotein P24 (IC50=0.71 nM), HIV-RT (IC50=0.76 nM).
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
-
Cytotoxicity
-
- Not included yet
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
-
Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP00333.
Physicochemical Information
-
Formula
- C278H444N82O88
Absent Amino Acids
- CMW
Common Amino Acids
- S
Mass
- 6601.77
PI
- 8.54
Basic Residues
- 7
Acidic Residues
- 5
Hydrophobic Residues
- 20
Net Charge
- +2
-
Boman Index
- -125.96
Hydrophobicity
- -0.253
Aliphatic Index
- 91
Half Life
-
- Mammalian:4.4 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 2980
Absorbance 280nm
- 50.51
Polar Residues
- 22
DRAMP00333
Comments Information
Function
- Single-chain ribosome-inactivating protein, possessing high antiviral potency and low toxicity to normal cells in culture and to intact animals. Capable of inhibiting HIV-1 infection and replication.
Catalytic activity
- Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Literature Information
- ·Literature 1
-
Title
- A new class of anti-HIV agents: GAP31, DAPs 30 and 32.
-
Pubmed ID
- 1936243
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Reference
- FEBS Lett. 1991 Oct 7;291(1):139-144.
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Author
- Lee-Huang S, Kung HF, Huang PL, Huang PL, Li BQ, Huang P, Huang HI, Chen HC.