General Information

    • DRAMP ID

    • DRAMP00335

    • Peptide Name

    • Ribosome-inactivating protein (rRNA N-glycosidase; Plant defensin)

    • Source

    • Cucurbita pepo (Vegetable marrow) (Summer squash)

    • Family

    • Belongs to the ribosome-inactivating protein family (Type 1 RIP subfamily)

    • Gene

    • Not found

    • Sequence

    • NVRFDLSGATSSSYKTFIKN

    • Sequence Length

    • 20

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Unknown

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00335 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00335.

Physicochemical Information

    • Formula

    • C99H155N27O32

    • Absent Amino Acids

    • CEHMPQW

    • Common Amino Acids

    • S

    • Mass

    • 2235.48

    • PI

    • 9.7

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +2

    • Boman Index

    • -44.31

    • Hydrophobicity

    • -0.46

    • Aliphatic Index

    • 58.5

    • Half Life

      • Mammalian:1.4 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 78.42

    • Polar Residues

    • 10

DRAMP00335

DRAMP00335 chydropathy plot

Comments Information

    • Catalytic activity

    • Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S Rrna.

Literature Information

  • ·Literature 1

    • Title

    • Purification, characterization and subcellular localization of a type-1 ribosome-inactivating protein from the sarcocarp of Cucurbita pepo.

    • Reference

    • Eur J Biochem. 1996 Dec 15;242(3):585-591.

    • Author

    • Yoshinari S, Yokota S, Sawamoto H, Koresawa S, Tamura M, Endo Y.