General Information
-
DRAMP ID
- DRAMP00398
-
Peptide Name
- Defensin D2 (Ns-D2; Plant defensin)
-
Source
- Nigella sativa (Black cumin)
-
Family
- Belongs to the DEFL family
-
Gene
- Not found
-
Sequence
- KFCEKPSGTWSGVCGNSGACKDQCIRLEGAKHGSCNYKLPAHRCICYYEC
-
Sequence Length
- 50
-
UniProt Entry
- P86973
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
-
Target Organism
-
- Fungi: Aspergillus niger (IC50=3.5 µg/ml), Bipolaris sorokiniana (IC50=1.8 µg/ml), Fusarium oxysporum (IC50=5.3 µg/ml), Fusarium graminearum (IC50=6.9 µg/ml), Fusarium culmorum (IC50=6.9 µg/ml) and Botrytis cinerea (IC50=13.7 µg/ml).
- Gram-positive bacteria: Clavibacter michiganensis (1.5 cm) and Bacillus subtilis (1.3 cm);
- Gram-negative bacteria: Pseudomonas syringae (1.3 cm), Erwinia carotovora (0.7 cm) and Escherichia coli (1.4 cm).
- NOTE: Inhibition zone; Defensin concentration (11 µg in 50 μl).
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
-
Cytotoxicity
-
- Not included yet
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
-
Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Bridge
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP00398.
Physicochemical Information
-
Formula
- C233H359N69O70S8
Absent Amino Acids
- M
Common Amino Acids
- C
Mass
- 5503.31
PI
- 8.48
Basic Residues
- 9
Acidic Residues
- 4
Hydrophobic Residues
- 10
Net Charge
- +5
-
Boman Index
- -81.13
Hydrophobicity
- -0.494
Aliphatic Index
- 43
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 10470
Absorbance 280nm
- 213.67
Polar Residues
- 24
DRAMP00398
Comments Information
Function
- Antimicrobial peptide active against fungi, Gram-positive and Gram-negative bacteria. Has no effect on spore germination. Destroys spores in germinated conidia by disruption of cell walls and membranes in Aspergillus niger and B.sorokiniana. Causes vacuolization of germinated macro- and microconidia in Fusarium oxysporum, F.graminearum and F.culmorum. Strongly inhibits growth of P.infestans on potato tubers above concentrations of 3.4 µg/ml.
PTM
- Contains four disulfide bonds.
Literature Information
- ·Literature 1
-
Title
- Novel antifungal defensins from Nigella sativa L. seeds.
-
Pubmed ID
- 21144761
-
Reference
- Plant Physiol Biochem. 2011 Feb;49(2):131-137.
-
Author
- Rogozhin EA, Oshchepkova YI, Odintsova TI, Khadeeva NV, Veshkurova ON, Egorov TA, Grishin EV, Salikhov SI.