General Information
-
DRAMP ID
- DRAMP00417
-
Peptide Name
- Raphanus sativus Antifungal Protein 4 (Rs-AFP4; Plant defensin)
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Source
- Raphanus sativus (Radish)
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Family
- Belongs to the DEFL family
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Gene
- AFP4
-
Sequence
- QKLCERSSGTWSGVCGNNNACKNQCINLEGARHGSCNYIFPYHRCICYFPC
-
Sequence Length
- 51
-
Swiss_Prot Entry
- O24331
-
Evidence code
- Homology
Activity Information
-
Biological Activity
- Antimicrobial, Antifungal
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Target Organism
-
- Fungi: Alternaria brassicicola (MIC=5 µg/ml), Botrytis cinerea (MIC=9 µg/ml), Fusarium culmorum (MIC=11 µg/ml).
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Hemolytic Activity
-
- Not found
-
Cytotoxicity
-
- Not included yet
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
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Other Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Bridge
-
Structure Description
- Not found
-
PDB ID
- None
-
-
Physicochemical Information
-
Formula
- C243H367N75O72S8
Absent Amino Acids
- DM
Common Amino Acids
- C
Mass
- 6639.56
PI
- 8.21
Basic Residues
- 7
Acidic Residues
- 2
Hydrophobic Residues
- 11
-
Boman Index
- -90.82
Hydrophobicity
- -0.433
Aliphatic Index
- 47.84
Half Life
-
- Mammalian:0.8 hour
- Yeast:10 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 10470
Absorbance 280nm
- 209.4
Polar Residues
- 27
DRAMP00417

Comments Information
PTM
- Contains four disulfide bonds 4-51;15-36;21-45;25-47, and Pyrrolidone carboxylic acid at position 1.
Literature Information
- ·Literature 1
-
Title
- Small cysteine-rich antifungal proteins from radish: their role in host defense.
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Pubmed ID
- 7780308
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Reference
- Plant Cell. 1995 May;7(5):573-588.
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Author
- Terras FR, Eggermont K, Kovaleva V, Raikhel NV, Osborn RW, Kester A, Rees SB, Torrekens S, Van Leuven F, Vanderleyden J, et al.