General Information
-
DRAMP ID
- DRAMP00454
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Peptide Name
- Petunia hybrida defensin 1 (PhD1; Cys-rich; Plant defensin)
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Source
- Petunia hybrida (Petunia)
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Family
- Belongs to the DEFL family
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Gene
- D1
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Sequence
- ATCKAECPTWDSVCINKKPCVACCKKAKFSDGHCSKILRRCLCTKEC
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Sequence Length
- 47
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Swiss_Prot Entry
- Q8H6Q1
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Evidence code
- Protein level
Activity Information
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Biological Activity
- Antifungal, Antimicrobial
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Target Organism
-
- Fungi: Fusarium oxysporum (MIC=10 µg/ml), Botrytis cinerea (MIC=10 µg/ml).
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Hemolytic Activity
-
- Not found
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
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Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Other Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Combine helix and strand structure
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Structure Description
- PhD1 has the fold of the cysteine-stabilized alphabeta motif, consisting of an alpha-helix and a triple-stranded antiparallel beta-sheet, except that it contains a fifth disulfide bond from the first loop to the alpha-helix.
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PDB ID
- 1N4N resolved by NMR.
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-
-
1N4N-> 
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Physicochemical Information
-
Formula
- C217H363N65O63S10
Absent Amino Acids
- MQY
Common Amino Acids
- C
Mass
- 6031.98
PI
- 8.58
Basic Residues
- 11
Acidic Residues
- 4
Hydrophobic Residues
- 12
-
Boman Index
- -80.46
Hydrophobicity
- -0.211
Aliphatic Index
- 54.04
Half Life
-
- Mammalian:4.4 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 6125
Absorbance 280nm
- 133.15
Polar Residues
- 18
DRAMP00454

Comments Information
Function
- Plant defense peptide with antifungal activity.
Tissue specificity
- Petals.
Domain
- The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
Biophysicochemical properties
- pH dependence (Stable under extremes of pH); Temperature dependence (Stable under extremes of temperature).
PTM
- Contains five disulfide bonds 3-47; 7-23; 14-34; 20-41; 24-43.
Literature Information
- ·Literature 1
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Title
- Isolation and properties of floral defensins from ornamental tobacco and petunia.
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Pubmed ID
- 12644678#12846570
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Reference
- Plant Physiol. 2003 Mar;131(3):1283-1293.
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Author
- Lay FT, Brugliera F, Anderson MA.