• DRAMP ID

    • DRAMP00764
    • Peptide Name

    • Piceain 1 (Plants)
    • Source

    • Picea sitchensis
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • KSLRPRCWIKIKFRCKSLKF
    • Sequence Length

    • 20
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Not found
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
    • Target Organism

      • Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=9.38 µg/ml), S. aureus ATCC 43300 (MIC=9.38 µg/ml);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=18.75 µg/ml), Escherichia coli ML-35P (MIC=18.75 µg/ml), Escherichia coli 08040722 (CI) (MIC=75 µg/ml), Escherichia coli 08A866 (CI) (MIC=37.5 µg/ml), Pseudomonas aeruginosa PA01 (MIC=9.38 µg/ml), Pseudomonas aeruginosa ATCC 27853 (MIC=9.38 µg/ml), Pseudomonas aeruginosa 08031014 (CI) (MIC=37.5 µg/ml).
      • Yeast: Candida albicans ATCC 20032 (MIC=9.38 µg/ml).
    • Hemolytic Activity

      • [Ref.21644248] At the concentration of 0.0625 mg/ml, Piceain 1 induced human & rabbit red cell hemolysis of 0.7% & 1.5%; At the concentration of 0.125 mg/ml, Piceain 1 induced human & rabbit red cell hemolysis of 0.8% & 7.2%; At the concentration of 0.25 mg/ml, Piceain 1 induced human & rabbit red cell hemolysis of 5.9%&7.8%; At the concentration of 0.5 mg/ml, Piceain 1 induced human & rabbit red cell hemolysis of 7.6% & 9.5%; At the concentration of 1 mg/ml, Piceain 1 induced human & rabbit red cell hemolysis of 7.8% & 57.1%.
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Cyclic
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Free
    • Nonterminal Modifications and Unusual Amino Acids

    • There is one intra-molecular disulfide bridge formed by Cys7 and Cys15.
    • Stereochemistry

    • L
    • Structure

    • Beta strand (CD)
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP00764 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP00764.
    • Formula

    • C118H197N35O23S2
    • Absent Amino Acids

    • ADEGHMNQTVY
    • Common Amino Acids

    • K
    • Mass

    • 2538.2
    • PI

    • 11.13
    • Basic Residues

    • 8
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 7
    • Net Charge

    • +8
    • Boman Index

    • -48.78
    • Hydrophobicity

    • -0.495
    • Aliphatic Index

    • 78
    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 5625
    • Absorbance 280nm

    • 296.05
    • Polar Residues

    • 4

DRAMP00764

DRAMP00764 chydropathy plot
    • Function

    • Active against both Gram-postive and Gram-negative bacteria, fungi. It showed weak hemolysis activity against human and rabbit red cells.
    • PTM

    • Contains one disulfide bond.
  • ·Literature 1
    • Title

    • Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis.
    • Reference

    • J Pept Sci. 2011 Sep;17(9):627-631.
    • Author

    • Liu R, Mu L, Liu H, Wei L, Yan T, Chen M, Zhang K, Li J, You D, Lai R.