General Information

    • DRAMP ID

    • DRAMP00769

    • Peptide Name

    • ChaC4 (Chassatide C4; Plant defensin)

    • Source

    • Chassalia chartacea

    • Family

    • Belongs to the cyclotide family

    • Gene

    • Not found

    • Sequence

    • GASCGETCFTGICFTAGCSCNPWPTCTRN

    • Sequence Length

    • 29

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Anti-cancer

    • Target Organism

      • Gram-negative bacterium: Escherichia coli (MIC>80 µM);
      • Gram-positive bacteria: Staphylococcus aureus (MIC>80 µM), Staphylococcus epidermidis (MIC>80 µM).

    • Hemolytic Activity

      • [Ref.22467870] HD50=51.9 µM against human type A red blood cells.

    • Cytotoxicity

      • [Ref.22467870] Cytotoxicity: HeLa cells (IC50=9.8 µM).

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys 4 and Cys18, Cys8 and Cys20, Cys13 and Cys26 .

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00769 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00769.

Physicochemical Information

    • Formula

    • C122H183N35O41S6

    • Absent Amino Acids

    • DHKLMQVY

    • Common Amino Acids

    • C

    • Mass

    • 2988.36

    • PI

    • 5.96

    • Basic Residues

    • 1

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 6

    • Net Charge

    • 0

    • Boman Index

    • -26.39

    • Hydrophobicity

    • 0.1

    • Aliphatic Index

    • 20.34

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5875

    • Absorbance 280nm

    • 209.82

    • Polar Residues

    • 19

DRAMP00769

DRAMP00769 chydropathy plot

Comments Information

    • IC50=9.8 µM, HD50=51.9 µM.##Note

    • IC50 (concentration that gives a survival index of 50%) and HD50 (concentration that causes 50% lysis of red blood cells).

Literature Information

  • ·Literature 1

    • Title

    • Novel Cyclotides and Uncyclotides with Highly Shortened Precursors from Chassalia chartacea and Effects of Methionine Oxidation on Bioactivities.

    • Reference

    • J Biol Chem. 2012 May 18;287(21):17598-17607.

    • Author

    • Nguyen GK, Lim WH, Nguyen PQ, Tam JP.