General Information
-
DRAMP ID
- DRAMP00770
-
Peptide Name
- ChaC10 (Chassatide C10; Plant defensin)
-
Source
- Chassalia chartacea
-
Family
- Belongs to the cyclotide family
-
Gene
- Not found
-
Sequence
- GEYCGESCYLIPCFTPGCYCVSRQCVNKN
-
Sequence Length
- 29
-
UniProt Entry
- No entry found
-
Protein Existence
- Not found
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Anti-cancer
-
Target Organism
-
- Gram-negative bacterium: Escherichia coli (MIC>80 µM);
- Gram-positive bacteria: Staphylococcus aureus (MIC>80 µM), Staphylococcus epidermidis (MIC>80 µM).
-
Hemolytic Activity
-
- [Ref.22467870] HD50>25 µM against human type A red blood cells.
-
Cytotoxicity
-
- [Ref.22467870] Cytotoxicity: HeLa cells (IC50=5.0 µM).
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- No specific N-terminal
-
C-terminal Modification
- No specific C-terminal
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys 4 and Cys18, Cys8 and Cys20, Cys13 and Cys25 .
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP00770.
Physicochemical Information
-
Formula
- C137H206N36O43S6
Absent Amino Acids
- ADHMW
Common Amino Acids
- C
Mass
- 3237.72
PI
- 6.1
Basic Residues
- 2
Acidic Residues
- 2
Hydrophobic Residues
- 5
Net Charge
- 0
-
Boman Index
- -31.3
Hydrophobicity
- -0.069
Aliphatic Index
- 46.9
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 4845
Absorbance 280nm
- 173.04
Polar Residues
- 17
DRAMP00770
Comments Information
IC50=5.0 µM, HD50>25 µM.##Note
- IC50 (concentration that gives a survival index of 50%) and HD50 (concentration that causes 50% lysis of red blood cells).
Literature Information
- ·Literature 1
-
Title
- Novel Cyclotides and Uncyclotides with Highly Shortened Precursors from Chassalia chartacea and Effects of Methionine Oxidation on Bioactivities.
-
Pubmed ID
- 22467870
-
Reference
- J Biol Chem. 2012 May 18;287(21):17598-17607.
-
Author
- Nguyen GK, Lim WH, Nguyen PQ, Tam JP.