General Information
-
DRAMP ID
- DRAMP00795
-
Peptide Name
- Cliotide T1 (cT1; Plant defensin)
-
Source
- Clitoria ternatea (Butterfly pea)
-
Family
- Belongs to the cyclotide family
-
Gene
- Not found
-
Sequence
- GIPCGESCVFIPCITAAIGCSCKSKVCYRN
-
Sequence Length
- 30
-
UniProt Entry
- No entry found
-
Protein Existence
- Not found
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Anti-cancer
-
Target Organism
-
- [Ref.21596752]Gram-positive bacteria: Staphylococcus aureus ATCC12600, Enterococcus faecalis ATCC 47077 (less active);
- Gram-negative bacteria: Escherichia coli ATCC 700926 (MIC=1.1 µM), Pseudomonas aeruginosa ATCC 39018 (MIC=2.7 µM), Klebsiella pneumonia ATCC 13883 (MIC=4.7 µM).
-
Hemolytic Activity
-
- [Ref.21596752] HD50 = 7.1 μM against human red blood cells
-
Cytotoxicity
-
- [Ref.21596752] IC50=0.6 µM against HeLa cells.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- There are three disulfide bonds between Cys4 and Cys20, Cys8 and Cys22, Cys13 and Cys27.
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP00795.
Physicochemical Information
-
Formula
- C132H216N36O39S6
Absent Amino Acids
- DHLMQW
Common Amino Acids
- C
Mass
- 3123.74
PI
- 8.33
Basic Residues
- 3
Acidic Residues
- 1
Hydrophobic Residues
- 9
Net Charge
- +2
-
Boman Index
- -7.52
Hydrophobicity
- 0.657
Aliphatic Index
- 78
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 1865
Absorbance 280nm
- 64.31
Polar Residues
- 15
DRAMP00795
Comments Information
Function
- Cliotide T1 shows stronger antimicrobial activity against the Gram-negative bacteria than the Gram-positive bacteria. It also has hemolytic activity against human type A erythrocytes (HD50=7.1 µM) and cytotoxicity against HeLa Cells (IC50=0.6 µM).
[Note
- HD50 refers to the peptide concentration that causes 50% lysis of red blood cells; IC50 refers to the peptide concentration that causes 50% death of HeLa cells].
Literature Information
- ·Literature 1
-
Title
- Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of albumin-1 chain a and cyclotide domains in the Fabaceae family.
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Pubmed ID
- 21596752
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Reference
- J Biol Chem. 2011 Jul 8;286(27):24275-24287.
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Author
- Nguyen GK, Zhang S, Nguyen NT, Nguyen PQ, Chiu MS, Hardjojo A, Tam JP.