General Information
-
DRAMP ID
- DRAMP00800
-
Peptide Name
- Cycloviolin-B (Plant defensin)
-
Source
- Leonia cymosa (Sacha uba)
-
Family
- Belongs to the cyclotide family
-
Gene
- Not found
-
Sequence
- GTACGESCYVLPCFTVGCTCTSSQCFKN
-
Sequence Length
- 28
-
UniProt Entry
- P84638
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antiviral
-
Target Organism
-
- [Ref.18008336]Virus:HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=130 nM).
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
-
Cytotoxicity
-
- [Ref.18008336]CEM-SS cells:IC50=560 nM.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Cyclization (N termini to C termini)
-
C-terminal Modification
- Cyclization (N termini to C termini)
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys4 and Cys18, Cys8 and Cys20,Cys13 and Cys25.
-
Stereochemistry
- L
-
Structure
- Bridge
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP00800.
Physicochemical Information
-
Formula
- C120H185N31O41S6
Absent Amino Acids
- DHIMRW
Common Amino Acids
- C
Mass
- 2910.33
PI
- 5.96
Basic Residues
- 1
Acidic Residues
- 1
Hydrophobic Residues
- 6
Net Charge
- 0
-
Boman Index
- -13.89
Hydrophobicity
- 0.389
Aliphatic Index
- 38.21
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 1865
Absorbance 280nm
- 69.07
Polar Residues
- 18
DRAMP00800
Comments Information
Function
- Probably participates in a plant defense mechanism. Has anti-HIV activity.
PTM
- This is a cyclic peptide which may contain three disulfide bonds 4-18;8-20;13-25.
Literature Information
- ·Literature 1
-
Title
- Cycloviolins A-D, anti-HIV macrocyclic peptides from Leonia cymosa.
-
Pubmed ID
- 10813905
-
Reference
- J Org Chem. 2000 Jan 14;65(1):124-128.
-
Author
- Hallock YF, Sowder RC 2nd, Pannell LK, Hughes CB, Johnson DG, Gulakowski R, Cardellina JH 2nd, Boyd MR.
- ·Literature 2
-
Title
- Cyclotides as natural anti-HIV agents.
-
Pubmed ID
- 18008336
-
Reference
- Biopolymers. 2008;90(1):51-60.
-
Author
- Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.