General Information
-
DRAMP ID
- DRAMP00837
-
Peptide Name
- Cycloviolacin-Y5 (Plants)
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Source
- Viola yedoensis (Chinese herb)
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Family
- Belongs to the cyclotide family
-
Gene
- Not found
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Sequence
- GIPCAESCVWIPCTVTALVGCSCSDKVCYN
-
Sequence Length
- 30
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UniProt Entry
- No entry found
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Protein Existence
- Not found
Activity Information
-
Biological Activity
- Antimicrobial, Antiviral
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Target Organism
-
- [Ref.18618891]Effects: H. contortus( IC50=2.28μM, IC99=22.24μM) and T. colubriformis(IC50=2.40μM, IC99=10.97μM).
- [Ref.18008336]Virus:HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=40 nM).
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Hemolytic Activity
-
- [Ref:18081258] HD50=8.7 μM against human type A red blood cells.
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Cytotoxicity
-
- [Ref.18008336]CEM-SS cells:IC50=1760 nM.
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
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N-terminal Modification
- Cyclization (N termini to C termini)
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C-terminal Modification
- Cyclization (N termini to C termini)
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Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys4 and Cys21; Cys8 and Cys23; Cys13 and Cys28.
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Stereochemistry
- L
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Structure
- Bridge
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP00837.
Physicochemical Information
-
Formula
- C132H209N33O42S6
Absent Amino Acids
- FHMQR
Common Amino Acids
- C
Mass
- 3122.67
PI
- 4.37
Basic Residues
- 1
Acidic Residues
- 2
Hydrophobic Residues
- 10
Net Charge
- -1
-
Boman Index
- 3.23
Hydrophobicity
- 0.793
Aliphatic Index
- 84.33
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 7365
Absorbance 280nm
- 253.97
Polar Residues
- 15
DRAMP00837
Comments Information
Function
- Has anti-HIV activity. Cycloviolacin Y5 is the most hydrophobic of the cyclotides from V. yedoensis, and is more hemolytic than cycloviolacin Y1.
PTM
- Contains three disulfide bonds.
Literature Information
- ·Literature 1
-
Title
- Anti-HIV cyclotides from the Chinese medicinal herb Viola yedoensis.
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Pubmed ID
- 18081258
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Reference
- J Nat Prod. 2008 Jan;71(1):47-52.
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Author
- Wang CK, Colgrave ML, Gustafson KR, Ireland DC, Goransson U, Craik DJ.
- ·Literature 2
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Title
- Cyclotides as natural anti-HIV agents
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Pubmed ID
- 18008336
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Reference
- Biopolymers. 2008;90(1):51-60.
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Author
- David C Ireland, Conan K L Wang, Jennifer A Wilson, Kirk R Gustafson, David J Craik
- ·Literature 3
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Title
- The anthelmintic activity of the cyclotides: natural variants with enhanced activity
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Pubmed ID
- 18618891
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Reference
- Chembiochem. 2008 Aug 11;9(12):1939-45. doi: 10.1002/cbic.200800174.
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Author
- Michelle L Colgrave, Andrew C Kotze, David C Ireland, Conan K Wang, David J Craik