• DRAMP ID

    • DRAMP00857
    • Peptide Name

    • Kalata-B2 (Plant defensin)
    • Source

    • Oldenlandia affinis
    • Family

    • Belongs to the cyclotide family
    • Gene

    • OAK4
    • Sequence

    • GLPVCGETCFGGTCNTPGCSCTWPICTRD
    • Sequence Length

    • 29
    • Protein Existence

    • Protein level
    • Biological Activity

    • Insecticidal
    • Target Organism

      • [Ref.17534989]Insects: Helicoverpa armigera
    • Hemolytic Activity

      • [Ref.20564013] HD50=3.3±1.9 μM against Human red blood cells .
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Cyclic
    • N-terminal Modification

    • No specific N-terminal
    • C-terminal Modification

    • No specific C-terminal
    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys5 and Cys19,Cys9 and Cys21,Cys14 and Cys26.
    • Stereochemistry

    • L
    • Structure

    • Bridge
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP00857 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP00857.
    • Formula

    • C122H188N34O41S6
    • Absent Amino Acids

    • AHKMQY
    • Common Amino Acids

    • C
    • Mass

    • 2979.4
    • PI

    • 4.37
    • Basic Residues

    • 1
    • Acidic Residues

    • 2
    • Hydrophobic Residues

    • 5
    • Net Charge

    • -1
    • Boman Index

    • -21.77
    • Hydrophobicity

    • 0.114
    • Aliphatic Index

    • 36.9
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 5875
    • Absorbance 280nm

    • 209.82
    • Polar Residues

    • 18

DRAMP00857

DRAMP00857 chydropathy plot
    • Function

    • Probably participates in a plant defense mechanism. Inhibitory effect on the growth and development of Helicoverpa armigera larvae suggests a role for the cyclotides in plant defense. Has hemolytic activity.
    • PTM

    • Kalata-B2 is a cyclic peptide which contains three disulfide bonds 5-19; 9-21; 14-26.
  • ·Literature 1
    • Title

    • Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif.
    • Reference

    • J Mol Biol. 1999 Dec 17;294(5):1327-1336.
    • Author

    • Craik DJ, Daly NL, Bond T, Waine C.
  • ·Literature 2
    • Title

    • Isolation, solution structure, and insecticidal activity of kalata B2, a circular protein with a twist: do Möbius strips exist in nature?
    • Reference

    • Biochemistry. 2005 Jan 25;44(3):851-860.
    • Author

    • Jennings CV, Rosengren KJ, Daly NL, Plan M, Stevens J, Scanlon MJ, Waine C, Norman DG, Anderson MA, Craik DJ.
  • ·Literature 3
    • Title

    • The cyclotide fingerprint in oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides.
    • Reference

    • Chembiochem. 2007 Jun 18;8(9):1001-1011.
    • Author

    • Plan MR, Göransson U, Clark RJ, Daly NL, Colgrave ML, Craik DJ.
  • ·Literature 4
    • Title

    • Structural and biochemical characteristics of the cyclotide kalata B5 from Oldenlandia affinis
    • Reference

    • Biopolymers . 2010;94(5):647-58.
    • Author

    • Manuel R Plan, K Johan Rosengren, Lillian Sando, Norelle L Daly, David J Craik