General Information

    • DRAMP ID

    • DRAMP00860

    • Peptide Name

    • Kalata-B5 (Plant defensin)

    • Source

    • Oldenlandia affinis

    • Family

    • Belongs to the cyclotide family

    • Gene

    • Not found

    • Sequence

    • TPCGESCVYIPCISGVIGCSCTDKVCYLN

    • Sequence Length

    • 29

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Not found

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • [Ref.20564013] HD50=27.4±1.2 μM against Human red blood cells .

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys4 and Cys20; Cys8 and Cys22; Cys13 and Cys27.Hydrogen bonds between the side chain carboxyl group of Glu6 and the backbone amide protons of Ile14 and Ser15, as well as the side chain hydroxyl proton of Ser15.

    • Stereochemistry

    • L

    • Structure

    • Combine helix and strand structure

    • Structure Description

    • It displays a typical tightly folded cyclic Scystine knot structure. A range of pH and temperature titrations reveal that a conserved glutamic acid in loop 1 Sof the structure forms a key hydrogen bond network, similar to that reported previously for other cyclotides.

    • Helical Wheel Diagram

    • DRAMP00860 helical wheel diagram

    • PDB ID

    • 2KUX resolved by NMR.
  • 2KUX-> 

    • Predicted Structure

    • There is no predicted structure for DRAMP00860.

Physicochemical Information

    • Formula

    • C127H203N31O42S6

    • Absent Amino Acids

    • AFHMQRW

    • Common Amino Acids

    • C

    • Mass

    • 3028.55

    • PI

    • 4.37

    • Basic Residues

    • 1

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 7

    • Net Charge

    • -1

    • Boman Index

    • -1.04

    • Hydrophobicity

    • 0.679

    • Aliphatic Index

    • 83.79

    • Half Life

      • Mammalian:7.2 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 3355

    • Absorbance 280nm

    • 119.82

    • Polar Residues

    • 17

DRAMP00860

DRAMP00860 chydropathy plot

Comments Information

    • Function

    • Probably participates in a plant defense mechanism.

    • PTM

    • Kalata-B5 is a cyclic peptide which contains three disulfide bonds 4-20; 8-22; 13-27.

Literature Information

  • ·Literature 1

    • Title

    • Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif.

    • Reference

    • J Mol Biol. 1999 Dec 17;294(5):1327-1336.

    • Author

    • Craik DJ, Daly NL, Bond T, Waine C.
  • ·Literature 2

    • Title

    • The cyclotide fingerprint in oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides.

    • Reference

    • Chembiochem. 2007 Jun 18;8(9):1001-1011.

    • Author

    • Plan MR, Göransson U, Clark RJ, Daly NL, Colgrave ML, Craik DJ.
  • ·Literature 3

    • Title

    • Structural and biochemical characteristics of the cyclotide kalata B5 from Oldenlandia affinis.

    • Reference

    • Biopolymers. 2010;94(5):647-658.

    • Author

    • Plan MR, Rosengren KJ, Sando L, Daly NL, Craik DJ.